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   Articles with "molecule localization" as a keyword



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Single‐molecule localization microscopy goes quantitative

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recommendations! Published in 2023 at "Microscopy Research and Technique"

DOI: 10.1002/jemt.24281

Abstract: In the last few years, single‐molecule localization (SMLM) techniques have been used to address biological questions in different research fields. More recently, super‐resolution has also been proposed as a quantitative tool for quantifying protein copy… read more here.

Keywords: localization microscopy; single molecule; molecule localization; microscopy ... See more keywords
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Single Molecule Localization Microscopy for Studying Small Extracellular Vesicles.

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recommendations! Published in 2023 at "Small"

DOI: 10.1002/smll.202205030

Abstract: Small extracellular vesicles (sEVs) are 30-200 nm nanovesicles enriched with unique cargoes of nucleic acids, lipids, and proteins. sEVs are released by all cell types and have emerged as a critical mediator of cell-to-cell communication. Although… read more here.

Keywords: localization microscopy; single molecule; extracellular vesicles; microscopy ... See more keywords
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Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons.

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recommendations! Published in 2022 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-2051-9_16

Abstract: Over the past years several forms of superresolution fluorescence microscopy have been developed that offer the possibility to study cellular structures and protein distribution at a resolution well below the diffraction limit of conventional fluorescence… read more here.

Keywords: microscopy; molecule localization; protein distribution; single molecule ... See more keywords
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Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion.

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recommendations! Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7759-8_15

Abstract: Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination… read more here.

Keywords: single molecule; microscopy; illumination microscopy; structured illumination ... See more keywords
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Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors

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recommendations! Published in 2019 at "Biophysical Reviews"

DOI: 10.1007/s12551-019-00595-2

Abstract: In the last decades, a promising breakthrough in fluorescence imaging was represented by the advent of super-resolution microscopy (SRM). Super-resolution techniques recently became a popular method to study sub-cellular structures, providing a successful approach to… read more here.

Keywords: study; single molecule; molecule localization; microscopy ... See more keywords
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Optical diffraction tomography from single-molecule localization microscopy

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recommendations! Published in 2021 at "Optics Communications"

DOI: 10.1016/j.optcom.2021.127290

Abstract: Abstract Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved positions from the… read more here.

Keywords: localization microscopy; single molecule; microscopy; molecule localization ... See more keywords
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About samples, giving examples: Optimized Single Molecule Localization Microscopy.

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recommendations! Published in 2019 at "Methods"

DOI: 10.1016/j.ymeth.2019.05.008

Abstract: Super-resolution microscopy has profoundly transformed how we study the architecture of cells, revealing unknown structures and refining our view of cellular assemblies. Among the various techniques, the resolution of Single Molecule Localization Microscopy (SMLM) can… read more here.

Keywords: localization microscopy; single molecule; molecule localization; microscopy ... See more keywords
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Single Molecule Localization Microscopy at Janelia's Advanced Imaging Center

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recommendations! Published in 2020 at "Microscopy and Microanalysis"

DOI: 10.1017/s1431927620018619

Abstract: Single molecule localization microscopy (SMLM) was initially demonstrated nearly 15 years ago in a trio of seminal papers. Since then, it has been recognized an integral method among the broader superresolution imaging repertoire available to… read more here.

Keywords: localization microscopy; single molecule; microscopy; molecule localization ... See more keywords
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Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities

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recommendations! Published in 2022 at "Nano Letters"

DOI: 10.1021/acs.nanolett.2c03140

Abstract: Single-molecule localization microscopy (SMLM) is a powerful technique for elucidating structure and dynamics in the life- and material sciences with sub-50 nm spatial resolution. The simultaneous acquisition of spectral information (spectrally resolved SMLM, sSMLM) enables… read more here.

Keywords: single molecule; localization; emitter; microscopy ... See more keywords
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vLUME: 3D virtual reality for single-molecule localization microscopy

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recommendations! Published in 2020 at "Nature Methods"

DOI: 10.1038/s41592-020-0962-1

Abstract: v LUME is a virtual reality software package designed to render large three-dimensional single-molecule localization microscopy datasets. v LUME features include visualization, segmentation, bespoke analysis of complex local geometries and exporting features. v LUME can… read more here.

Keywords: single molecule; microscopy; virtual reality; localization microscopy ... See more keywords
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Time-correlated single molecule localization microscopy enhances resolution and fidelity

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recommendations! Published in 2020 at "Scientific Reports"

DOI: 10.1038/s41598-020-72812-y

Abstract: Single-molecule-localization-microscopy (SMLM) enables superresolution imaging of biological samples down to ~ 10–20 nm and in single molecule detail. However, common SMLM reconstruction largely disregards information embedded in the entire intensity trajectories of individual emitters. Here, we… read more here.

Keywords: single molecule; microscopy; time correlated; localization microscopy ... See more keywords