Full-length codling moth transcriptome atlas revealed by single-molecule real-time sequencing.
Sign Up to like & getrecommendations! Published in 2022 at "Genomics"
DOI: 10.1016/j.ygeno.2022.110299
Abstract: Over the past decade, second-generation sequencing (SGS) has been widely used to elucidate the transcriptome across many organisms. However, the full-length (FL) transcripts and alternative splice (AS) isoforms could not be confidently and accurately defined… read more here.
Keywords: molecule real; codling moth; real time; full length ... See more keywords
The value of single-molecule real-time technology in the diagnosis of rare thalassemia variants and analysis of phenotype-genotype correlation.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of human genetics"
DOI: 10.1038/s10038-021-00983-1
Abstract: To compare single-molecule real-time technology (SMRT) and conventional genetic diagnostic technology of rare types of thalassemia mutations, and to analyze the molecular characteristics and phenotypes of rare thalassemia gene variants, we used 434 cases with… read more here.
Keywords: rare thalassemia; single molecule; real time; molecule real ... See more keywords
Construction of JRG (Japanese reference genome) with single-molecule real-time sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Human Genome Variation"
DOI: 10.1038/s41439-019-0057-7
Abstract: In recent genome analyses, population-specific reference panels have indicated important. However, reference panels based on short-read sequencing data do not sufficiently cover long insertions. Therefore, the nature of long insertions has not been well documented.… read more here.
Keywords: single molecule; reference genome; molecule real; reference ... See more keywords
Bacterial microbiota composition of fermented fruit and vegetable juices (jiaosu) analyzed by single-molecule, real-time (SMRT) sequencing
Sign Up to like & getrecommendations! Published in 2018 at "CyTA - Journal of Food"
DOI: 10.1080/19476337.2018.1512531
Abstract: ABSTRACT Commercially manufactured ‘jiaosu’ (fermented fruit and vegetable juices) have gained popularity in Asia recently. Like other fermented products, they have a high microbial diversity and richness. However, no published study has yet described their… read more here.
Keywords: single molecule; fermented fruit; molecule real; fruit vegetable ... See more keywords
Application of the Single-Molecule Real-Time Technology (SMRT) for Identification of HKαα Thalassemia Allele
Sign Up to like & getrecommendations! Published in 2022 at "Laboratory Medicine"
DOI: 10.1093/labmed/lmac065
Abstract: Abstract Objective Single-molecule real-time technology (SMRT) is a sequencing technology using the DNA polymerases and fluorescently tagged nucleotides to accurately sequence DNA strands. The purpose of this study was to evaluate the detection accuracy of… read more here.
Keywords: single molecule; time technology; technology; thalassemia ... See more keywords
SARS-CoV-2 Co-Infections and Recombinations Identified by Long-Read Single-Molecule Real-Time Sequencing
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00493-23
Abstract: SARS-CoV-2 variants have been responsible for the successive waves of infection over the 3 years of pandemic. While co-infection followed by recombination is one driver of virus evolution, there have been few reports of co-infections,… read more here.
Keywords: single molecule; omicron variants; real time; molecule real ... See more keywords
Single-Molecule Real-Time Sequencing to Explore the Mycobiome Diversity in Malt
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00511-22
Abstract: Fungal contamination and secondary metabolite accumulation in agricultural products represent a global food safety challenge. Although high-throughput sequencing (HTS) is beneficial for explaining fungal communities, it presents disadvantages, such as short reads, species-level resolution, and… read more here.
Keywords: single molecule; fungal communities; diversity; real time ... See more keywords
Single-molecule real-time sequencing of the full-length transcriptome of loquat under low-temperature stress
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0238942
Abstract: In this study, third-generation full-length (FL) transcriptome sequencing was performed of loquat using single-molecule real-time(SMRT) sequencing from the pooled cDNA of embryos of young loquat fruit under different low temperatures (three biological replicates for treatments… read more here.
Keywords: loquat; single molecule; full length; length transcriptome ... See more keywords
Integrated Analysis of Single-Molecule Real-Time Sequencing and Next-Generation Sequencing Eveals Insights into Drought Tolerance Mechanism of Lolium multiflorum
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23147921
Abstract: Lolium multiflorum is widely planted in temperate and subtropical regions globally, and it has high economic value owing to its use as forage grass for a wide variety of livestock and poultry. However, drought seriously… read more here.
Keywords: single molecule; lolium multiflorum; analysis; real time ... See more keywords
Bisulfite treatment and single-molecule real-time sequencing reveal D-loop length, position, and distribution
Sign Up to like & getrecommendations! Published in 2020 at "eLife"
DOI: 10.7554/elife.59111
Abstract: Displacement loops (D-loops) are signature intermediates formed during homologous recombination. Numerous factors regulate D-loop formation and disruption, thereby influencing crucial aspects of DNA repair, including donor choice and the possibility of crossover outcome. While D-loop… read more here.
Keywords: bisulfite treatment; length position; single molecule; loop ... See more keywords