In cellulo FRET-FLIM and single molecule tracking reveal the supra-molecular organization of the pyoverdine bio-synthetic enzymes in Pseudomonas aeruginosa
Sign Up to like & getrecommendations! Published in 2020 at "Quarterly Reviews of Biophysics"
DOI: 10.1017/s0033583519000155
Abstract: Abstract The bio-synthesis of pyoverdine (PVD) in Pseudomonas aeruginosa involves multiple enzymatic steps including the action of non-ribosomal peptide synthetases (NRPSs). One hallmark of NRPS is their ability to make usage of non-proteinogenic amino-acids synthesized… read more here.
Keywords: single molecule; supra molecular; molecule tracking; bio ... See more keywords
Single-Molecule Tracking of Reagent Diffusion during Chemical Reactions.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c13172
Abstract: Recent experiments have shown that the diffusion of reagent molecules is inconsistent with what the Stokes-Einstein equation predicts during a chemical reaction. Here, we used single-molecule tracking to observe the diffusion of reactive reagent molecules… read more here.
Keywords: single molecule; reagent diffusion; diffusion; reaction ... See more keywords
Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function
Sign Up to like & getrecommendations! Published in 2018 at "Nature Chemical Biology"
DOI: 10.1038/s41589-018-0032-5
Abstract: AbstractSingle-fluorescent-molecule imaging tracking (SMT) is becoming an important tool to study living cells. However, photobleaching and photoblinking (hereafter referred to as photobleaching/photoblinking) of the probe molecules strongly hamper SMT studies of living cells, making it… read more here.
Keywords: molecule; single molecule; photobleaching photoblinking; super long ... See more keywords
Super-long single-molecule tracking
Sign Up to like & getrecommendations! Published in 2018 at "Nature Methods"
DOI: 10.1038/s41592-018-0026-y
Abstract: Biocompatible, bio-orthogonal chemical reactions such as the Staudinger ligation have proven to be useful methods for biochemical and imaging studies in living cells, as well as for drug discovery. Wang et al. now add sulfur–fluoride… read more here.
Keywords: single molecule; long single; chemistry; super long ... See more keywords
Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-34572-8
Abstract: Single-particle (molecule) tracking (SPT/SMT) is a powerful method to study dynamic processes in living bacterial cells at high spatial and temporal resolution. We have performed single-molecule imaging of early DNA double-strand break (DSB) repair events… read more here.
Keywords: dna repair; dna; molecule tracking; repair centres ... See more keywords
Single molecule tracking and analysis framework including theory-predicted parameter settings
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-88802-7
Abstract: Imaging, tracking and analyzing individual biomolecules in living systems is a powerful technology to obtain quantitative kinetic and spatial information such as reaction rates, diffusion coefficients and localization maps. Common tracking tools often operate on… read more here.
Keywords: single molecule; tracking analysis; framework; analysis ... See more keywords
The needle and the haystack: single molecule tracking to probe the transcription factor search in eukaryotes
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Society Transactions"
DOI: 10.1042/bst20200709
Abstract: Transcription factors (TFs) regulate transcription of their target genes by identifying and binding to regulatory regions of the genome among billions of potential non-specific decoy sites, a task that is often presented as a ‘needle… read more here.
Keywords: single molecule; transcription; molecule tracking; needle haystack ... See more keywords
Single-molecule tracking (SMT): a window into live-cell transcription biochemistry
Sign Up to like & getrecommendations! Published in 2023 at "Biochemical Society Transactions"
DOI: 10.1042/bst20221242
Abstract: How molecules interact governs how they move. Single-molecule tracking (SMT) thus provides a unique window into the dynamic interactions of biomolecules within live cells. Using transcription regulation as a case study, we describe how SMT… read more here.
Keywords: single molecule; transcription; molecule tracking; tracking smt ... See more keywords