Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Asia-Pacific Entomology"
DOI: 10.1016/j.aspen.2019.02.008
Abstract: Abstract Reverse transcriptase from Moloney murine leukemia virus (MMLVRT) is an RNA dependent DNA polymerase, which has been used as a fundamental tool for molecular biology and biotechnology. The secondary structures formed in the RNA… read more here.
Keywords: expression; system; reverse transcriptase; moloney murine ... See more keywords
Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep41769
Abstract: Moloney murine leukemia virus reverse transcriptase (MMLV-RT) is a widely used enzyme for cDNA synthesis. Here we show that MMLV-RT has a strong template-independent polymerase activity using blunt DNA ends as substrate that generates 3′… read more here.
Keywords: moloney murine; dna; murine leukemia; dna ends ... See more keywords