Monitoring Macromolecular Crowding via FRET using Wavelength-Dependent Fluorescence Lifetime Measurements
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DOI: 10.1016/j.bpj.2016.11.1885
Abstract: Macromolecular crowding influences many intracellular processes such as protein folding, biochemical reactions and diffusion. Recently, a rationally designed FRET probe was developed to quantify macromolecular crowding in vitro (Boersma et al. Nat. Meth. 12:227, 2015).… read more here.
Keywords: monitoring macromolecular; fluorescence lifetime; macromolecular crowding; lifetime measurements ... See more keywords