Engineering a Protein Binder Specific for p38α with Interface Expansion.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00408
Abstract: Protein binding specificities can be manipulated by redesigning contacts that already exist at an interface or by expanding the interface to allow interactions with residues adjacent to the original binding site. Previously, we developed a… read more here.
Keywords: protein; engineering protein; monobody; protein binder ... See more keywords
Targeted Protein Degradation through Cytosolic Delivery of Monobody Binders Using Bacterial Toxins
Sign Up to like & getrecommendations! Published in 2019 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.9b00113
Abstract: Monobodies are small engineered binding proteins that, upon expression in cells, can inhibit signaling of cytosolic oncoproteins with outstanding selectivity. Efficacy may be further increased by inducing degradation of monobody targets through fusion to the… read more here.
Keywords: cytosolic delivery; monobody; delivery; protein degradation ... See more keywords
Facile target validation in an animal model with intracellularly expressed monobodies
Sign Up to like & getrecommendations! Published in 2018 at "Nature chemical biology"
DOI: 10.1038/s41589-018-0099-z
Abstract: Rapidly determining the biological effect of perturbing a site within a potential drug target could guide drug discovery efforts, but it remains challenging. Here, we describe a facile target validation approach that exploits monobodies, small… read more here.
Keywords: monobody; validation; target; facile target ... See more keywords
Engineering of monobody conjugates for human EphA2-specific optical imaging
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0180786
Abstract: In a previous study, we developed an E1 monobody specific for the tumor biomarker hEphA2 [PLoS ONE (2015) 10(7): e0132976]. E1 showed potential as a molecular probe for in vitro and in vivo targeting of… read more here.
Keywords: monobody; rluc8; optical imaging; specific optical ... See more keywords