Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest‐like precursors
Sign Up to like & getrecommendations! Published in 2017 at "Febs Letters"
DOI: 10.1002/1873-3468.12572
Abstract: In this study, we propose a novel method for inducing neuronal cells by briefly exposing them to small‐molecule cocktails in a step‐by‐step manner. Global gene expression analysis with immunohistochemical staining and calcium flux assays reveal… read more here.
Keywords: embryonic fibroblasts; brief exposure; exposure small; crest like ... See more keywords
Cathepsin A regulates pluripotency, proliferation and differentiation in mouse embryonic stem cells
Sign Up to like & getrecommendations! Published in 2020 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.3554
Abstract: Mouse embryonic stem cells (mESCs) are pluripotent cells that possess the ability to self‐renew and differentiate into three germ layers. Owing to these characteristics, mESCs act as important models for stem cell research and are… read more here.
Keywords: proliferation; differentiation; mouse embryonic; embryonic stem ... See more keywords
Epidermal growth factor and three-dimensional scaffolds provide conducive environment for differentiation of mouse embryonic stem cells into oocyte-like cells.
Sign Up to like & getrecommendations! Published in 2020 at "Cell biology international"
DOI: 10.1002/cbin.11391
Abstract: Three-dimensional (3D) culture provides a biomimicry of the naive microenvironment that can support cell proliferation, differentiation and regeneration. Some growth factors, such as epidermal growth factor (EGF), facilitate normal meiosis during oocyte maturation in vivo.… read more here.
Keywords: growth factor; oocyte like; differentiation; mouse embryonic ... See more keywords
Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/7651_2021_438
Abstract: With their unique capabilities of self-renewal and differentiation into three germ layers, mouse embryonic stem cells (mESCs) are widely used as an in vitro cellular model for early mammalian developmental studies. mESCs are traditionally cultured… read more here.
Keywords: cell culture; serum; stem cell; mouse embryonic ... See more keywords
Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells Through Protein Transduction of Transcription Factors.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0943-9_7
Abstract: In this chapter, we describe a simple and unique method for the differentiation of mouse embryonic stem cells into insulin-producing cells. In addition to cytokines and growth factors, key transcription factors for pancreatic development are… read more here.
Keywords: transcription factors; producing cells; mouse embryonic; embryonic stem ... See more keywords
Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1601-7_1
Abstract: Forced expression of specific neuronal transcription factors in mouse embryonic fibroblasts (MEFs) can lead to their direct conversion into functional neurons. Direct neuronal reprogramming has become a powerful tool to characterize individual factors and molecular… read more here.
Keywords: isolation neuronal; embryonic fibroblasts; mouse embryonic; reprogramming ... See more keywords
Directed Differentiation of Mouse Embryonic Stem Cells Into Inner Ear Sensory Epithelia in 3D Culture.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6949-4_6
Abstract: The inner ear sensory epithelium harbors mechanosensory hair cells responsible for detecting sound and maintaining balance. This protocol describes a three-dimensional (3D) culture system that efficiently generates inner ear sensory epithelia from aggregates of mouse… read more here.
Keywords: inner ear; sensory epithelia; mouse embryonic; ear sensory ... See more keywords
The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8669-9_9
Abstract: The introduction of stem cell-based technologies for the derivation of three-dimensional retinal tissues, the so-called retinal organoids, offers many new possibilities for vision research: Organoids facilitate studies on retinal development and in vitro retinal disease… read more here.
Keywords: retinal organoid; embryonic stem; mouse embryonic; retinal organoids ... See more keywords
Propagation and Maintenance of Mouse Embryonic Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9086-3_3
Abstract: Mouse embryonic stem cells (mESCs) are pluripotent cells derived from preimplantation embryos that have the capacity to self-renew indefinitely in vitro. mESCs are an indispensable tool for studying cellular differentiation in vitro, generating disease in… read more here.
Keywords: propagation maintenance; mouse embryonic; embryonic stem; stem cells ... See more keywords
Gene Editing of Mouse Embryonic and Epiblast Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9086-3_6
Abstract: Efficient and reliable methods for gene editing are critical for the generation of loss-of-gene function stem cells and genetically modified mice. Here, we outline the application of CRISPR-Cas9 technology for gene editing in mouse embryonic… read more here.
Keywords: gene editing; editing mouse; mouse embryonic; gene ... See more keywords
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
Sign Up to like & getrecommendations! Published in 2017 at "Diabetologia"
DOI: 10.1007/s00125-017-4302-7
Abstract: Aims/hypothesisPancreatic beta-like cells generated from human induced pluripotent stem cells (hiPSCs) or human embryonic stem cells (hESCs) offer an appealing donor tissue source. However, differentiation protocols that mainly use growth factors are costly. Therefore, in… read more here.
Keywords: embryonic pancreatic; differentiation; mouse embryonic; endocrine ... See more keywords