Non‐ubiquitous expression of core spliceosomal protein SmB/B′ in chick and mouse embryos
Sign Up to like & getrecommendations! Published in 2022 at "Developmental Dynamics"
DOI: 10.1002/dvdy.537
Abstract: Although splicing is an integral part of the expression of many genes in our body, genetic syndromes with spliceosomal defects affect only specific tissues. To help understand the mechanism, we investigated the expression pattern of… read more here.
Keywords: core; expression; chick mouse; mouse embryos ... See more keywords
Regulation of Kit Expression in Early Mouse Embryos and ES Cells
Sign Up to like & getrecommendations! Published in 2019 at "STEM CELLS"
DOI: 10.1002/stem.2960
Abstract: Kit is a growth factor receptor that regulates proliferation and/or survival of many embryonic and postnatal stem cell types. When mutated, it can induce malignant transformation of the host cells. To dissect the Kit role… read more here.
Keywords: egfp expression; kit; expression; expression early ... See more keywords
Chromatin Immunoprecipitation in Early Mouse Embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7714-7_14
Abstract: Epigenetic regulation is achieved at many levels by different factors such as tissue-specific transcription factors, members of the basal transcriptional apparatus, chromatin-binding proteins, and noncoding RNAs. Importantly, chromatin structure dictates the availability of a specific… read more here.
Keywords: chromatin; mouse embryos; chromatin immunoprecipitation; mouse ... See more keywords
Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7714-7_4
Abstract: The heart is a dynamic organ that quickly undergoes morphological and mechanical changes through early embryonic development. Characterizing these early moments is important for our understanding of proper embryonic development and the treatment of heart… read more here.
Keywords: mouse embryos; heart; development; dynamic imaging ... See more keywords
Ex Utero Culture and Imaging of Mouse Embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9009-2_11
Abstract: Mouse genetic approaches when combined with live imaging tools are revolutionizing our current understanding of mammalian developmental biology. The availability and improvement of a wide variety of genetically encoded fluorescent proteins have provided indispensable tools… read more here.
Keywords: utero culture; culture imaging; biology; mouse embryos ... See more keywords
Effect of low temperatures on cytochrome photoresponse in mouse embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2019.05.017
Abstract: Embryos cryopreservation is a widely used technology for genetic resources storage. Cryopreservation suppresses cell respiration, but very little is known about the changes that occur with mitochondria at low temperatures. We used Raman spectroscopy to… read more here.
Keywords: mouse embryos; low temperatures; cytochrome photoresponse; spectroscopy ... See more keywords
Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.11.039
Abstract: In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET, is transiently and asymmetrically… read more here.
Keywords: cell; cell fate; cell mouse; mouse embryos ... See more keywords
Both Nodal signalling and stochasticity select for prospective distal visceral endoderm in mouse embryos
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01625-x
Abstract: Anterior–posterior (A–P) polarity of mouse embryos is established by distal visceral endoderm (DVE) at embryonic day (E) 5.5. Lefty1 is expressed first at E3.5 in a subset of epiblast progenitor cells (L1epi cells) and then… read more here.
Keywords: endoderm; distal visceral; visceral endoderm; mouse embryos ... See more keywords
Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-02706-7
Abstract: Recent advances using CRISPR-Cas9 approaches have dramatically enhanced the ease for genetic manipulation in rodents. Notwithstanding, the methods to deliver nucleic acids into pre-implantation embryos have hardly changed since the original description of mouse transgenesis… read more here.
Keywords: embryos; crispr cas9; recombinant adeno; adeno associated ... See more keywords
Immunofluorescent Staining of Whole-Mount Mouse Embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot094037
Abstract: Mouse embryos and fetal organs have no pigmentation except for the retinal pigmented epithelium of the eye at subsequent stages of development. This makes it difficult to visualize and photodocument embryonic structures using conventional light… read more here.
Keywords: whole mount; microscopy; staining whole; mouse embryos ... See more keywords
Intravital imaging of mouse embryos
Sign Up to like & getrecommendations! Published in 2020 at "Science"
DOI: 10.1126/science.aba0210
Abstract: A window to the embryo Mammalian embryonic development is a complex process, continuously changing in space and time. Q. Huang et al. designed an abdominal window to image mouse embryos in utero from embryonic day… read more here.
Keywords: window; ncc derived; embryonic day; day birth ... See more keywords