Dry ice is a reliable substrate for the distribution of frozen mouse spermatozoa: A multi-centric study.
Sign Up to like & getrecommendations! Published in 2017 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2017.04.003
Abstract: Disseminating mouse stocks as frozen materials offers both ethical and logistical advantages over live animal shipment, minimizing the welfare issues and avoiding some of the complex custom regulations that are associated with live animal transportation.… read more here.
Keywords: centric study; dry ice; multi centric; mouse spermatozoa ... See more keywords
Long term maintenance of frozen mouse spermatozoa at -80 °C.
Sign Up to like & getrecommendations! Published in 2018 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2017.10.036
Abstract: Maintaining mouse stocks as frozen materials offers both ethical and economical advantages over live animal breeding if the lines are not actively used. The European Mouse Mutant Archive (EMMA) promotes the archiving and distribution of… read more here.
Keywords: frozen mouse; spermatozoa; maintenance; long term ... See more keywords
Analysis of the small non-protein-coding RNA profile of mouse spermatozoa reveals specific enrichment of piRNAs within mature spermatozoa
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DOI: 10.1080/15476286.2017.1356569
Abstract: ABSTRACT Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa possess a complex small non-protein-coding RNA (sRNA) profile, the composition… read more here.
Keywords: spermatozoa; non protein; small non; mouse spermatozoa ... See more keywords
The impact of five years storage/biobanking at −80°C on mouse spermatozoa fertility, physiology, and function
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DOI: 10.1111/andr.12971
Abstract: We previously demonstrated how mouse spermatozoa can be efficiently stored for two years in a −80°C freezer, maintaining their ability to fertilize mouse eggs. read more here.
Keywords: years storage; impact five; five years; physiology ... See more keywords
MRP4-mediated cAMP efflux is essential for mouse spermatozoa capacitation
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DOI: 10.1242/jcs.230565
Abstract: ABSTRACT Mammalian spermatozoa must undergo biochemical and structural changes to acquire the capacity for fertilization, in a process known as capacitation. Activation of PKA enzymes is essential for capacitation, and thus cAMP levels are tightly… read more here.
Keywords: camp efflux; capacitation; mrp4; mouse spermatozoa ... See more keywords