Application of Phosphoramidate ProTide Technology for the Synthesis of 5’‐mRNA Cap Analogs Modified on the Exocyclic Amine Group
Sign Up to like & getrecommendations! Published in 2023 at "ChemMedChem"
DOI: 10.1002/cmdc.202200490
Abstract: Aryloxy triester phosphoramidate methodology, commonly known as ProTide technology, is one of the most widely used prodrug approaches applied to therapeutic nucleosides. This approach has been used extensively by the pharmaceutical industry and researchers in… read more here.
Keywords: cap; synthesis; protide technology; mrna cap ... See more keywords
Deciphering the mechanistic effects of eIF4E phosphorylation on mRNA‐cap recognition
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DOI: 10.1002/pro.3798
Abstract: The mRNA cap‐binding oncoprotein “eIF4E” is phosphorylated at residue S209 by Mnk kinases, and is closely associated with tumor development and progression. Despite being well‐established, mechanistic details at the molecular level of mRNA recognition by… read more here.
Keywords: recognition; eif4e phosphorylation; mrna cap; cap ... See more keywords
Isoxazole-containing 5' mRNA cap analogues as inhibitors of the translation initiation process.
Sign Up to like & getrecommendations! Published in 2020 at "Bioorganic chemistry"
DOI: 10.1016/j.bioorg.2020.103583
Abstract: Herein we describe a synthesis of new isoxazole-containing 5' mRNA cap analogues via a cycloaddition reaction. The obtained analogues show a capability to inhibit cap-dependent translation in vitro and are characterized by a new binding… read more here.
Keywords: isoxazole containing; mrna cap; containing mrna; cap analogues ... See more keywords
Quantification of mRNA cap-modifications by means of LC-QqQ-MS.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.05.018
Abstract: Enzymatic modification of the 5'-cap is a versatile approach to modulate the properties of mRNAs. Transfer of methyl groups from S-adenosyl-L-methionine (AdoMet) or functional moieties from non-natural analogs by methyltransferases (MTases) allows for site-specific modifications… read more here.
Keywords: mrna cap; modifications means; cap modifications; quantification mrna ... See more keywords
Capping Enzyme mRNA-cap/RNGTT Regulates Hedgehog Pathway Activity by Antagonizing Protein Kinase A
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DOI: 10.1038/s41598-017-03165-2
Abstract: Hedgehog (Hh) signaling plays a pivotal role in animal development and its deregulation in humans causes birth defects and several types of cancer. Protein Kinase A (PKA) modulates Hh signaling activity through phosphorylating the transcription… read more here.
Keywords: mrna cap; capping enzyme; pathway; activity ... See more keywords
c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical Journal"
DOI: 10.1042/bcj20160930
Abstract: The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNA guanine-7 methyltransferase (RNMT)–RNA-activating miniprotein (RAM), the mRNA cap… read more here.
Keywords: rna; mrna cap; rnmt ram; cap ... See more keywords
2′-O-Methylation of the second transcribed nucleotide within the mRNA 5′ cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac722
Abstract: In higher eukaryotes, m7G-adjacent nucleotides undergo extensive modifications. Ribose of the first or first and second transcribed nucleotides can be subjected to 2’-O-methylation to form cap1 or cap2, respectively. Additionally, when the first transcribed nucleotide… read more here.
Keywords: methylation; methylation second; transcribed nucleotide; mrna cap ... See more keywords