Yeast Puf3p-mediated mRNA decay is regulated by carbon source-specific differential interaction of Puf3p with Pop2p and Yak1p.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS letters"
DOI: 10.1002/1873-3468.14624
Abstract: Puf3p regulates the stability of nuclear-encoded mRNAs acting in mitochondrial biogenesis and function in Saccharomyces cerevisiae. This work identifies the phosphorylation of Pop2p, a component of the deadenylase complex, as being critical for adapting Puf3p-mediated… read more here.
Keywords: yak1p; puf3p mediated; puf3p; mrna decay ... See more keywords
SMG1, a nonsense‐mediated mRNA decay (NMD) regulator, as a candidate therapeutic target in multiple myeloma
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13343
Abstract: Early data suggested that CC‐115, a clinical molecule, already known to inhibit the mammalian target of rapamycin kinase (TORK) and DNA‐dependent protein kinase (DNA‐PK) may have additional targets beyond TORK and DNA‐PK. Therefore, we aimed… read more here.
Keywords: nonsense mediated; smg1; decay nmd; mrna decay ... See more keywords
Somatic MED12 Nonsense Mutation Escapes mRNA Decay and Reveals a Motif Required for Nuclear Entry
Sign Up to like & getrecommendations! Published in 2017 at "Human Mutation"
DOI: 10.1002/humu.23157
Abstract: MED12 is a key component of the transcription‐regulating Mediator complex. Specific missense and in‐frame insertion/deletion mutations in exons 1 and 2 have been identified in uterine leiomyomas, breast tumors, and chronic lymphocytic leukemia. Here, we… read more here.
Keywords: nonsense mutation; somatic med12; med12; mrna decay ... See more keywords
Phase‐separated bacterial ribonucleoprotein bodies organize mRNA decay
Sign Up to like & getrecommendations! Published in 2020 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1599
Abstract: In bacteria, mRNA decay is controlled by megadalton scale macromolecular assemblies called, “RNA degradosomes,” composed of nucleases and other RNA decay associated proteins. Recent advances in bacterial cell biology have shown that RNA degradosomes can… read more here.
Keywords: ribonucleoprotein bodies; phase separated; mrna decay; bacterial ribonucleoprotein ... See more keywords
Variation of the response to metal ions and nonsense‐mediated mRNA decay across different Saccharomyces cerevisiae genetic backgrounds
Sign Up to like & getrecommendations! Published in 2021 at "Yeast"
DOI: 10.1002/yea.3565
Abstract: Regulation of mRNA steady‐state levels is important in controlling gene expression particularly in response to environmental stimuli. This allows cells to rapidly respond to environment changes. The highly conserved nonsense‐mediated mRNA decay (NMD) pathway was… read more here.
Keywords: saccharomyces cerevisiae; response; genetic backgrounds; mediated mrna ... See more keywords
Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq).
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7540-2_13
Abstract: Recent progress in the technology of transcriptome-wide high-throughput sequencing has revealed that nonsense-mediated mRNA decay (NMD) targets ~10% of physiologic transcripts for the purpose of tuning gene expression in response to various environmental conditions. Regardless… read more here.
Keywords: decay nmd; phosphorylated upf1; mediated mrna; nmd targets ... See more keywords
Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7540-2_4
Abstract: Cellular mRNA levels are determined by the rates of mRNA synthesis and mRNA decay. Typically, mRNA degradation kinetics are measured on a population of cells that are either chemically treated or genetically engineered to inhibit… read more here.
Keywords: mrna decay; decay; single molecule; yeast ... See more keywords
Orchestration of Processing Body Dynamics and mRNA Decay in Arabidopsis Immunity.
Sign Up to like & getrecommendations! Published in 2019 at "Cell reports"
DOI: 10.1016/j.celrep.2019.07.054
Abstract: Proper transcriptome reprogramming is critical for hosts to launch an effective defense response upon pathogen attack. How immune-related genes are regulated at the posttranscriptional level remains elusive. We demonstrate here that P-bodies, the non-membranous cytoplasmic… read more here.
Keywords: orchestration processing; body dynamics; immunity; processing body ... See more keywords
Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis.
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.107542
Abstract: RNA-binding proteins (RBPs) play a pivotal role in gene expression by modulating the stability of transcripts. However, the identification of degradation target mRNAs of RBPs remains difficult. By the combined analysis of transcriptome-wide mRNA stabilities… read more here.
Keywords: dna damage; analysis; mrna decay; mediated mrna ... See more keywords
New insights into no-go, non-stop and nonsense-mediated mRNA decay complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2020.06.011
Abstract: Eukaryotes possess a variety of translational control mechanisms which function in the surveillance of mRNAs, discriminating between normal and aberrant translation elongation and termination, triggering mRNA decay. The three major evolutionarily conserved eukaryotic pathways are… read more here.
Keywords: non stop; mrna decay; mediated mrna; nonsense mediated ... See more keywords
Inhibiting transcription in cultured metazoan cells with actinomycin D to monitor mRNA turnover.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.01.003
Abstract: Decay of transcribed mRNA is a key determinant of steady state mRNA levels in cells. Global analysis of mRNA decay in cultured cells has revealed amazing heterogeneity in rates of decay under normal growth conditions,… read more here.
Keywords: mrna decay; decay rates; inhibiting transcription; mrna ... See more keywords