'Head-to-Head' mRNA display for the translation of multi-copied proteins with a free C-terminus.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.07.015
Abstract: With the development of various methods for affinity-based selection of proteins such as phage display, ribosomal display, and mRNA display, the progress in this field has been gradually shifting to function-based selection, such as through… read more here.
Keywords: mrna display; head head; multi copied; display ... See more keywords
Potent Cyclic Peptide Inhibitors of FXIIa Discovered by mRNA Display with Genetic Code Reprogramming.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.1c00651
Abstract: The contact system comprises a series of serine proteases that mediate procoagulant and proinflammatory activities via the intrinsic pathway of coagulation and the kallikrein-kinin system, respectively. Inhibition of Factor XIIa (FXIIa), an initiator of the… read more here.
Keywords: mrna display; cyclic peptide; inhibitors fxiia; peptide inhibitors ... See more keywords
mRNA Display Discovery of a Novel Programmed Death Ligand 1 (PD-L1) Binding Peptide (a Peptide Ligand for PD-L1).
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.0c00264
Abstract: Programmed death ligand 1 (PD-L1) is a critical immune checkpoint ligand whose overexpression on tumor cells provides a mechanism of escape from immune surveillance. The interaction between PD-L1 and PD-1 on T cell lymphocytes suppresses… read more here.
Keywords: peptide; mrna display; programmed death; death ligand ... See more keywords
Directed Evolution of PD-L1-Targeted Affibodies by mRNA Display.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00218
Abstract: Therapeutic monoclonal antibodies directed against PD-L1 (e.g., atezolizumab) disrupt PD-L1:PD-1 signaling and reactivate exhausted cytotoxic T-cells in the tumor compartment. Although anti-PD-L1 antibodies are successful as immune checkpoint inhibitor (ICI) therapeutics, there is still a… read more here.
Keywords: amino acids; display; affinity; mrna display ... See more keywords
Enzymatic Macrolactamization of mRNA Display Libraries for Inhibitor Selection.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00828
Abstract: mRNA display is a powerful, high-throughput technology for discovering novel, peptide ligands for protein targets. A number of methods have been used to expand the chemical diversity of mRNA display libraries beyond the 20 canonical… read more here.
Keywords: display; mrna display; display libraries; cyclization ... See more keywords
Direct, Competitive Comparison of Linear, Monocyclic, and Bicyclic Libraries Using mRNA Display.
Sign Up to like & getrecommendations! Published in 2020 at "ACS combinatorial science"
DOI: 10.1021/acscombsci.0c00016
Abstract: Peptide macrocyclization is typically associated with the development of higher affinity and more protease stable protein ligands, and, as such, is an important tool in peptide drug discovery. Yet, within the context of a diverse… read more here.
Keywords: comparison linear; mrna display; linear monocyclic; direct competitive ... See more keywords
Tyrosinase-Catalyzed Peptide Macrocyclization for mRNA Display.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c12629
Abstract: mRNA display of macrocyclic peptides has proven itself to be a powerful technique to discover high-affinity ligands for a protein target. However, only a limited number of cyclization chemistries are known to be compatible with… read more here.
Keywords: mrna display; mrna; display tyrosinase; tyrosinase catalyzed ... See more keywords
A new strategy for the in vitro selection of stapled peptide inhibitors by mRNA display.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c8cc10192b
Abstract: Hydrocarbon stapled peptides are promising therapeutics for inhibition of intracellular protein-protein interactions. Here we develop a new high-throughput strategy for hydrocarbon stapled peptide discovery based on mRNA display of peptides containing α-methyl cysteine and cyclized… read more here.
Keywords: stapled peptide; vitro selection; mrna display; strategy vitro ... See more keywords