A bicistronic vector with destabilized mRNA secondary structure yields scalable higher titer expression of human neurturin in E. coli
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DOI: 10.1002/bit.26299
Abstract: Human neurturin (NTN) is a cystine knot growth factor with potential therapeutic use in diseases such as Parkinson's and diabetes. Scalable high titer production of native NTN is particularly challenging because of the cystine knot… read more here.
Keywords: bicistronic vector; secondary structure; mrna secondary; expression ... See more keywords
On the role of mRNA secondary structure in bacterial translation
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DOI: 10.1002/wrna.1579
Abstract: Messenger RNA (mRNA) is no longer considered as a mere informational molecule whose sole function is to convey the genetic information specified by DNA to the ribosome. Beyond this primary function, mRNA also contains additional… read more here.
Keywords: secondary structure; mrna secondary; translation; structure bacterial ... See more keywords
Secondary Structure of Chloroplast mRNAs In Vivo and In Vitro
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DOI: 10.3390/plants9030323
Abstract: mRNA secondary structure can influence gene expression, e.g., by influencing translation initiation. The probing of in vivo mRNA secondary structures is therefore necessary to understand what determines the efficiency and regulation of gene expression. Here,… read more here.
Keywords: vivo vitro; chloroplast; mrna secondary; secondary structure ... See more keywords