Retroelement origins of pre‐mRNA splicing
Sign Up to like & getrecommendations! Published in 2020 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1589
Abstract: Recent cryo‐EM structures of a group II intron caught in the process of invading DNA have given new insight into the mechanisms of both splicing and retrotransposition. Conformational dynamics involving the branch‐site helix domain VI… read more here.
Keywords: group; origins pre; mrna splicing; pre mrna ... See more keywords
Design and In Vitro Use of Antisense Oligonucleotides to Correct Pre-mRNA Splicing Defects in Inherited Retinal Dystrophies.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7522-8_5
Abstract: Antisense oligonucleotides (AONs) are small molecules able to bind to the pre-mRNA and modulate splicing. The increasing amount of intronic mutations leading to pseudoexon insertion in genes underlying inherited retinal dystrophies (IRDs) has highlighted the… read more here.
Keywords: correct pre; retinal dystrophies; mrna splicing; pre mrna ... See more keywords
An Ancient Pseudoknot in TNF-α Pre-mRNA Activates PKR, Inducing eIF2α Phosphorylation that Potently Enhances Splicing.
Sign Up to like & getrecommendations! Published in 2017 at "Cell reports"
DOI: 10.1016/j.celrep.2017.06.035
Abstract: Tumor necrosis factor alpha (TNF-α) is expressed promptly during inflammatory responses. Efficient TNF-α mRNA splicing is achieved through a 3' UTR element that activates RNA-dependent eIF2α protein kinase (PKR). The TNF-α RNA activator, we show,… read more here.
Keywords: enhances splicing; potently enhances; mrna splicing; eif2 phosphorylation ... See more keywords
A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex.
Sign Up to like & getrecommendations! Published in 2021 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2021.02.007
Abstract: Dysregulated pre-mRNA splicing is an emerging Achilles heel of cancers and myelodysplasias. To expand the currently limited portfolio of small-molecule drug leads, we screened for chemical modulators of the U2AF complex, which nucleates spliceosome assembly… read more here.
Keywords: small molecule; mrna splicing; pre mrna; u2af2 rna ... See more keywords
Multiplex PCR and NGS-based identification of mRNA splicing variants: Analysis of BRCA1 splicing pattern as a model.
Sign Up to like & getrecommendations! Published in 2017 at "Gene"
DOI: 10.1016/j.gene.2017.09.025
Abstract: Alternative pre-mRNA splicing increases transcriptome plasticity by forming naturally-occurring alternative splicing variants (ASVs). Alterations of splicing processes, caused by DNA mutations, result in aberrant splicing and the formation of aberrant mRNA isoforms. Analyses of hereditary… read more here.
Keywords: analysis brca1; mrna splicing; splicing variants; multiplex pcr ... See more keywords
Specific intron-dependent loading of DAZAP1 onto the cox6c transcript suppresses pre-mRNA splicing efficacy and induces cell growth retardation.
Sign Up to like & getrecommendations! Published in 2018 at "Gene"
DOI: 10.1016/j.gene.2018.03.005
Abstract: DAZAP1 is an evolutionarily conserved RNA-binding protein expressed in many tissues in mice and humans. DAZAP1-knockout mice carrying a partial loss-of-function (hypomorphic) allele exhibited severe deficiencies in spermatogenesis and cell growth, indicating that DAZAP1 plays… read more here.
Keywords: cox6c; cell growth; mrna splicing; dazap1 ... See more keywords
A comparison of alternative mRNA splicing in the CD4 and CD8 T cell lineages.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular immunology"
DOI: 10.1016/j.molimm.2021.02.009
Abstract: T cells can be subdivided into a number of different subsets that are defined by their distinct functions. While the specialization of different T cell subsets is partly achieved by the expression of specific genes,… read more here.
Keywords: alternative mrna; cd4 cd8; mrna splicing; cell ... See more keywords
F9 mRNA splicing aberration due to a deep Intronic structural variation in a patient with moderate hemophilia B.
Sign Up to like & getrecommendations! Published in 2022 at "Thrombosis research"
DOI: 10.1016/j.thromres.2022.03.010
Abstract: INTRODUCTION Hemophilia B (HB) is a hereditary bleeding disorder caused by the genetic variation of the coagulation factor IX (FIX) gene (F9). Several F9 structural abnormalities, including large deletion and/or insertion, have been observed to… read more here.
Keywords: mrna splicing; deep intronic; patient moderate; variation ... See more keywords
Emerging Roles of Ubiquitin-like Proteins in Pre-mRNA Splicing.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2018.09.001
Abstract: Ubiquitin-like proteins (UBLs) belong to the protein family whose members share a globular beta-grasp fold structure. The archetypal member, ubiquitin, is known for its function in proteasome-mediated protein degradation. UBLs have been shown to play… read more here.
Keywords: like proteins; ubiquitin like; emerging roles; mrna splicing ... See more keywords
Understanding pre-mRNA splicing through crystallography.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.04.023
Abstract: Crystallography is a powerful tool to determine the atomic structures of proteins and RNAs. X-ray crystallography has been used to determine the structure of many splicing related proteins and RNAs, making major contributions to our… read more here.
Keywords: mrna splicing; understanding pre; crystallography; pre mrna ... See more keywords
Multiplexed primer extension sequencing: A targeted RNA-seq method that enables high-precision quantitation of mRNA splicing isoforms and rare pre-mRNA splicing intermediates.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.05.013
Abstract: The study of pre-mRNA splicing has been greatly aided by the advent of RNA sequencing (RNA-seq), which enables the genome-wide detection of discrete splice isoforms. Quantification of these splice isoforms requires analysis of splicing informative… read more here.
Keywords: mrna splicing; rna seq; pre mrna; splice isoforms ... See more keywords