Manipulation of mRNA translation elongation influences the fragmentation of a biotherapeutic Fc‐fusion protein produced in CHO cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28230
Abstract: Mammalian cells, particularly Chinese hamster ovary cells, are the dominant system for the production of protein‐based biotherapeutics, however, product degradation, particularly of Fc‐fusion proteins, is sometimes observed that impacts the quality of the protein generated.… read more here.
Keywords: fusion; translation elongation; fusion protein; mrna translation ... See more keywords
Sevoflurane impairs m6A-mediated mRNA translation and leads to fine motor and cognitive deficits.
Sign Up to like & getrecommendations! Published in 2021 at "Cell biology and toxicology"
DOI: 10.1007/s10565-021-09601-4
Abstract: Clinical surgical practices have found that children who undergo multiple anesthesia may have an increased risk of deficiencies in cognition and fine motor control. Here, we report that YT521-B homology domain family 1 (YTHDF1), a… read more here.
Keywords: anesthesia; mrna translation; motor; fine motor ... See more keywords
Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2018.11.030
Abstract: Summary Local translation regulates the axonal proteome, playing an important role in neuronal wiring and axon maintenance. How axonal mRNAs are localized to specific subcellular sites for translation, however, is not understood. Here we report… read more here.
Keywords: translation platforms; mrna translation; translation; late endosomes ... See more keywords
Targeting mRNA translation in Parkinson's disease.
Sign Up to like & getrecommendations! Published in 2019 at "Drug discovery today"
DOI: 10.1016/j.drudis.2019.04.003
Abstract: Parkinson's disease (PD) is one of the most common neurodegenerative disorders. The exact cause(s) of PD is not well understood, although genetic mutations are associated with some forms of the disease. Many of these mutations,… read more here.
Keywords: targeting mrna; mrna translation; translation; parkinson disease ... See more keywords
Mitochondrial mRNA translation initiation contributes to oxidative metabolism in the myocardia of aged, obese mice
Sign Up to like & getrecommendations! Published in 2019 at "Experimental Gerontology"
DOI: 10.1016/j.exger.2019.03.009
Abstract: Abstract Being both advanced in age and obese each contribute to cardiac hypertrophy in a unique manner. Electron transport complexes I and IV are implicated in deficient electron transport during cardiomyopathies and contain the majority… read more here.
Keywords: obese mice; translation initiation; mrna translation; aged obese ... See more keywords
A Requirement for Mena, an Actin Regulator, in Local mRNA Translation in Developing Neurons
Sign Up to like & getrecommendations! Published in 2017 at "Neuron"
DOI: 10.1016/j.neuron.2017.06.048
Abstract: During neuronal development, local mRNA translation is required for axon guidance and synaptogenesis, and dysregulation of this process contributes to multiple neurodevelopmental and cognitive disorders. However, regulation of local protein synthesis in developing axons remains poorly… read more here.
Keywords: actin; mena; mrna translation; translation ... See more keywords
Regulation of mRNA Translation in Neurons—A Matter of Life and Death
Sign Up to like & getrecommendations! Published in 2017 at "Neuron"
DOI: 10.1016/j.neuron.2017.09.057
Abstract: Dynamic regulation of mRNA translation initiation and elongation is essential for the survival and function of neural cells. Global reductions in translation initiation resulting from mutations in the translational machinery or inappropriate activation of the… read more here.
Keywords: regulation mrna; death; mrna translation; translation ... See more keywords
An epitranscriptomic mechanism underlies selective mRNA translation remodelling in melanoma persister cells
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-13360-6
Abstract: Cancer persister cells tolerate anticancer drugs and serve as the founders of acquired resistance and cancer relapse. Here we show that a subpopulation of BRAFV600 mutant melanoma cells that tolerates exposure to BRAF and MEK… read more here.
Keywords: melanoma; persister cells; persister; mrna translation ... See more keywords
Quantification of mRNA translation in live cells using single-molecule imaging
Sign Up to like & getrecommendations! Published in 2020 at "Nature Protocols"
DOI: 10.1038/s41596-019-0284-x
Abstract: mRNA translation is a key step in gene expression. Proper regulation of translation efficiency ensures correct protein expression levels in the cell, which is essential to cell function. Different methods used to study translational control… read more here.
Keywords: microscopy; live cells; mrna translation; translation ... See more keywords
Upregulation of 5′-terminal oligopyrimidine mRNA translation upon loss of the ARF tumor suppressor
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-79379-8
Abstract: Tumor cells require nominal increases in protein synthesis in order to maintain high proliferation rates. As such, tumor cells must acquire enhanced ribosome production. How the numerous mutations in tumor cells ultimately achieve this aberrant… read more here.
Keywords: arf; oligopyrimidine mrna; mrna translation; translation ... See more keywords
Single-molecule mRNA and translation imaging in neurons.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Society transactions"
DOI: 10.1042/bst20210313
Abstract: Memory-relevant neuronal plasticity is believed to require local translation of new proteins at synapses. Understanding this process has necessitated the development of tools to visualize mRNA within relevant neuronal compartments. In this review, we summarize… read more here.
Keywords: mrna translation; single molecule; translation; molecule mrna ... See more keywords