A Biosilification Fusion Protein for a ‘Self‐immobilising’ Sarcosine Oxidase Amperometric Enzyme Biosensor
Sign Up to like & getrecommendations! Published in 2020 at "Electroanalysis"
DOI: 10.1002/elan.202000032
Abstract: Monomeric sarcosine oxidase (mSOx) fusion with the silaffin peptide, R5, designed previously for easy protein production in low resource areas, was used in a biosilification process to form an enzyme layer electrode biosensor. mSOx is… read more here.
Keywords: biosilification; sarcosine oxidase; biosensor; enzyme ... See more keywords
Design of a H2O2‐generating P450SPα fusion protein for high yield fatty acid conversion
Sign Up to like & getrecommendations! Published in 2022 at "Protein Science"
DOI: 10.1002/pro.4501
Abstract: Sphingomonas paucimobilis' P450SPα (CYP152B1) is a good candidate as industrial biocatalyst. This enzyme is able to use hydrogen peroxide as unique cofactor to catalyze the fatty acids conversion to α‐hydroxy fatty acids, thus avoiding the… read more here.
Keywords: fusion protein; conversion; h2o2; yield ... See more keywords
Gene to diagnostic: Self immobilizing protein for silica microparticle biosensor, modelled with sarcosine oxidase.
Sign Up to like & getrecommendations! Published in 2019 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2018.12.003
Abstract: A rational design approach is proposed for a multifunctional enzyme reagent for point-of-care diagnostics. The biomaterial reduces downstream isolation steps and eliminates immobilization coupling chemicals for integration in a diagnostic platform. Fusion constructs combined the… read more here.
Keywords: protein; sarcosine oxidase; silica; particle ... See more keywords