Detection of DNA methyltransferase activity using template-free DNA polymerization amplification based on aggregation-induced emission.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.113532
Abstract: A sensitive and selective fluorescence assay for DNA methyltransferase (MTase) activity detection was designed based on aggregation-induced emission (AIE) and target initiated template-free DNA polymerization. Quaternized tetraphenylethene salt was synthesized as the AIE probe, which… read more here.
Keywords: detection; dna; methyltransferase; mtase ... See more keywords
Fluorescence biosensor for DNA methyltransferase activity and related inhibitor detection based on methylation-sensitive cleavage primer triggered hyperbranched rolling circle amplification.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.04.061
Abstract: Highly sensitive and selective detection of DNA adenine methylation methyltransferase (Dam MTase) activity is essential for clinical diagnosis and treatment as Dam MTase can catalyze DNA methylation and has a profound effect on gene regulation.… read more here.
Keywords: methylation; detection; dam mtase; fluorescence ... See more keywords
Optimizing peptide inhibitors of SARS-Cov-2 nsp10/nsp16 methyltransferase predicted through molecular simulation and machine learning
Sign Up to like & getrecommendations! Published in 2022 at "Informatics in Medicine Unlocked"
DOI: 10.1016/j.imu.2022.100886
Abstract: Coronaviruses, including the recent pandemic strain SARS-Cov-2, use a multifunctional 2′-O-methyltransferase (2′-O-MTase) to restrict the host defense mechanism and to methylate RNA. The nonstructural protein 16 2′-O-MTase (nsp16) becomes active when nonstructural protein 10 (nsp10)… read more here.
Keywords: sars cov; methyltransferase; optimizing peptide; peptide inhibitors ... See more keywords
A cascade strand displacement amplification strategy for highly sensitive and label-free detection of DNA methyltransferase activity
Sign Up to like & getrecommendations! Published in 2021 at "Microchemical Journal"
DOI: 10.1016/j.microc.2020.105775
Abstract: Abstract DNA methyltransferase (MTase) is regarded as a promising biomarker and therapeutic target, which plays an important regulatory role in many biological processes. Here, a cascade strand displacement amplification strategy was explored for highly sensitive… read more here.
Keywords: strand displacement; detection; dna; displacement amplification ... See more keywords
An Isothermal Autocatalytic Hybridization Reaction Circuit for Sensitive Detection of DNA Methyltransferase and Inhibitors Assay.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00037
Abstract: Abnormal DNA methylation contributes to the annoying tumorigenesis and the elevated expression of methylation-related methyltransferase (MTase) is associated with many diseases. Hence DNA MTase could serve as a promising biomarker for cancer-specific diagnosis as well… read more here.
Keywords: mtase; reaction; circuit; hybridization ... See more keywords
Versatile Electrochemiluminescence and Electrochemical "On-Off" Assays of Methyltransferases and Aflatoxin B1 Based on a Novel Multifunctional DNA Nanotube.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b05362
Abstract: Herein, a new multifunctional DNA nanotube (DNANT) was self-assembled and used to load Ru(phen)32+ and methylene blue (MB) as amplified signal probes for versatile electrochemiluminescence (ECL) and electrochemical (EC) "on-off" assays of Dam methylase (MTase)… read more here.
Keywords: signal probes; dna nanotube; dna; multifunctional dna ... See more keywords
Highly Sensitive Assay of Methyltransferase Activity Based on an Autonomous Concatenated DNA Circuit.
Sign Up to like & getrecommendations! Published in 2018 at "ACS sensors"
DOI: 10.1021/acssensors.8b00738
Abstract: Methyltransferase-involved DNA methylation is one of the most important epigenetic processes, making the ultrasensitive MTase assay highly desirable in clinical diagnosis as well as biomedical research. Traditional single-stage amplification means often achieve linear amplification that… read more here.
Keywords: cha hcr; circuit; mtase; dna circuit ... See more keywords
Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00359
Abstract: Essential viral enzymes have been successfully targeted to combat the diseases caused by emerging pathogenic RNA viruses (e.g., viral RNA-dependent RNA polymerase). Because of the conserved nature of such viral enzymes, therapeutics targeting these enzymes… read more here.
Keywords: mtase; cov genome; rna; targeting sars ... See more keywords
RNA 2′-O-methylation modification and its implication in COVID-19 immunity
Sign Up to like & getrecommendations! Published in 2020 at "Cell Death Discovery"
DOI: 10.1038/s41420-020-00358-z
Abstract: The recent outbreak of a novel human coronavirus infection (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a serious threat to public health, which has currently led to more than 25 million… read more here.
Keywords: methylation; rna; host; sars cov ... See more keywords
Structural analysis of the SARS-CoV-2 methyltransferase complex involved in RNA cap creation bound to sinefungin
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17495-9
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the COVID-19 pandemic. 2′-O-RNA methyltransferase (MTase) is one of the enzymes of this virus that is a potential target for antiviral therapy as it… read more here.
Keywords: rna cap; methyltransferase; structural analysis; sars cov ... See more keywords
A glucometer-based strategy for sensitive DNA methyltransferase activity detection via a polymerization nicking reaction and enzyme amplification
Sign Up to like & getrecommendations! Published in 2017 at "Analytical Methods"
DOI: 10.1039/c7ay00712d
Abstract: DNA methyltransferase (MTase) is closely related to the DNA methylation level in many biological processes and has been recognized as a predictive cancer biomarker which occurs before other signs of malignancy and a therapeutic target… read more here.
Keywords: enzyme amplification; dna; strategy; mtase ... See more keywords