Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0868-5_11
Abstract: Budding yeast Saccharomyces cerevisiae has become a model eukaryotic microorganism for targeted genomic manipulation due to its efficient homologous recombination. A few genomic loci, including rDNA, Delta, and Ty1, can be utilized to introduce variable… read more here.
Keywords: copy; gate assembly; genetic elements; complex genetic ... See more keywords
Integrity Auditing for Multi-Copy in Cloud Storage Based on Red-Black Tree
Sign Up to like & getrecommendations! Published in 2021 at "IEEE Access"
DOI: 10.1109/access.2021.3079143
Abstract: With the rapid development of cloud storage, cloud users are willing to store data in the cloud storage system, and at the same time, the requirements for the security, integrity, and availability of data storage… read more here.
Keywords: based red; multi copy; tree; storage ... See more keywords
On multi-copy forwarding protocols for large data chunk dissemination in vehicular sensor networks
Sign Up to like & getrecommendations! Published in 2018 at "EURASIP Journal on Wireless Communications and Networking"
DOI: 10.1186/s13638-018-1139-9
Abstract: Moving vehicles have been sensing all kinds of data on the road in which multimedia data possesses a large portion. These data is often forwarded to vehicles in a region of interest or the monitoring… read more here.
Keywords: forwarding protocols; data chunk; copy; copy forwarding ... See more keywords
Developing Multi-Copy Chromosomal Integration Strategies for Heterologous Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.851706
Abstract: Caffeic acid, a plant-sourced phenolic compound, has a variety of biological activities, such as antioxidant and antimicrobial properties. The caffeic acid biosynthetic pathway was initially constructed in S. cerevisiae, using codon-optimized TAL (coTAL, encoding tyrosine… read more here.
Keywords: acid; multi copy; integration; caffeic acid ... See more keywords
Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation
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DOI: 10.3390/molecules27030800
Abstract: Chitosanase hydrolyzes β-(1,4)-linked glycosidic bonds are used in chitosan chains to release oligosaccharide mixtures. Here, we cloned and expressed a cold-adapted chitosanase (CDA, Genbank: MW094131) using multi-copy expression plasmids (CDA1/2/3/4) in Pichia pastoris. We identified… read more here.
Keywords: high density; cda; multi copy; expression ... See more keywords