mmquant: how to count multi-mapping reads?
Sign Up to like & getrecommendations! Published in 2017 at "BMC Bioinformatics"
DOI: 10.1186/s12859-017-1816-4
Abstract: BackgroundRNA-Seq is currently used routinely, and it provides accurate information on gene transcription. However, the method cannot accurately estimate duplicated genes expression. Several strategies have been previously used (drop duplicated genes, distribute uniformly the reads,… read more here.
Keywords: multi mapping; mapping reads; mmquant count; duplicated genes ... See more keywords
Probably Correct: Rescuing Repeats with Short and Long Reads
Sign Up to like & getrecommendations! Published in 2020 at "Genes"
DOI: 10.3390/genes12010048
Abstract: Ever since the introduction of high-throughput sequencing following the human genome project, assembling short reads into a reference of sufficient quality posed a significant problem as a large portion of the human genome—estimated 50–69%—is repetitive.… read more here.
Keywords: multi mapping; rescuing repeats; probably correct; long reads ... See more keywords