Expressing Multi-subunit Complexes Using biGBac.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7759-8_21
Abstract: The reconstitution of recombinant protein complexes is facilitated by methods that allow coexpression of their subunits from a single vector. Here we describe a detailed step-by-step protocol for the biGBac cloning method which can be… read more here.
Keywords: expressing multi; multi subunit; step; bigbac ... See more keywords
Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular cell"
DOI: 10.1016/j.molcel.2022.01.026
Abstract: CRISPR-Cas biology and technologies have been largely shaped to date by the characterization and use of single-effector nucleases. By contrast, multi-subunit effectors dominate natural systems, represent emerging technologies, and were recently associated with RNA-guided DNA transposition.… read more here.
Keywords: characterization multi; crispr; cell free; multi subunit ... See more keywords
Building ubiquitination machineries: E3 ligase multi-subunit assembly and substrate targeting by PROTACs and molecular glues.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2020.10.009
Abstract: E3 ubiquitin ligase machineries are emerging as attractive therapeutic targets because they confer specificity to substrate ubiquitination and can be hijacked for targeted protein degradation. In this review, we bring to focus our current structural… read more here.
Keywords: machineries ligase; ligase multi; ubiquitination machineries; multi subunit ... See more keywords
Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase
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DOI: 10.1038/ncomms15774
Abstract: Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a ∼400 kDa five-subunit catalytic core. Here we characterize a hypothetical ∼100 kDa single-chain protein, YonO,… read more here.
Keywords: multi subunit; polymerase; rna polymerase; subunit rna ... See more keywords
Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review
Sign Up to like & getrecommendations! Published in 2022 at "Iranian Journal of Parasitology"
DOI: 10.18502/ijpa.v17i3.10618
Abstract: Protein complexes are involved in many vital biological processes. Therefore, researchers need these protein complexes for biochemical and biophysical studies. Several methods exist for expressing multi-subunit proteins in eukaryotic cells, such as 2A sequences, IRES,… read more here.
Keywords: protein; subunit; polycistronic expression; multi subunit ... See more keywords