Multienzyme Cascade Synthesis of Rare Sugars From Glycerol in Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202400539
Abstract: Rare sugars are valuable and unique monosaccharides extensively utilized in the food, cosmetics, and pharmaceutical industries. Considering the high purification costs and the complex processes of enzymatic synthesis, whole‐cell conversion has emerged as a significantly… read more here.
Keywords: rare sugars; synthesis rare; cascade synthesis; synthesis ... See more keywords
Designing a Novel Multienzyme Cascade with a Coenzyme Self-Recycling System for the Synthesis of 2-Phenylethyl-β-d-glucopyranoside from Phenylalanine.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c02309
Abstract: Phenylethanoid glycosides (PhGs) are glycoconjugates composed of phenylethyl alcohols and glycoside moieties, exhibiting diverse bioactivities. 2-Phenylethyl-β-d-glucopyranoside (2PE-Glc) is the simplest form of PhGs. We successfully designed and implemented a novel multienzyme cascade system with cofactor… read more here.
Keywords: cascade system; system; 2pe glc; multienzyme cascade ... See more keywords
Structural Insights into Trehalose-6-Phosphate Phosphorylase and Its Role in Trehalose 6-Phosphate Biosynthesis via a Multienzyme Cascade.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c05090
Abstract: Trehalose 6-phosphate (Tre6P) is a critical metabolic signaling molecule in plants, orchestrating diverse biological processes, including stress resistance and photosynthetic efficiency. Recent advancements highlight its promising role in improving crop yield. However, the efficient synthesis… read more here.
Keywords: trehalose phosphate; phosphate phosphorylase; wctre6ppase; tre6p ... See more keywords
Efficient biosynthesis of high-value succinic acid and 5-hydroxyleucine using a multienzyme cascade and whole-cell catalysis.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.9b05529
Abstract: Succinic acid (SA), is applied in the food, chemical, and pharmaceutical industries. 5-Hydroxyleucine (5-HLeu) is a promising precursor for biosynthesis of antituberculosis drugs. Here, we designed a promising synthetic route for simultaneous production of SA… read more here.
Keywords: multienzyme cascade; succinic acid; whole cell; high value ... See more keywords
Multienzyme Cascade Catalyzed Skeleton Rearrangement in a Caged Polyketide Biosynthesis.
Sign Up to like & getrecommendations! Published in 2024 at "Organic letters"
DOI: 10.1021/acs.orglett.4c04412
Abstract: Rearrangement of the skeleton is crucial for improving the structural complexity and diversity of type II polyketide natural products. In this study, we investigated the rearrangement process from a planar aromatic tetracyclic intermediate to the… read more here.
Keywords: cascade catalyzed; catalyzed skeleton; skeleton rearrangement; rearrangement caged ... See more keywords
Targeting and Microenvironment-Activated Nanoreactor for Diabetic Chronic Wound Healing via Multienzyme Cascade Reactions.
Sign Up to like & getrecommendations! Published in 2024 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.3c12427
Abstract: The development of cell-like nanoreactors with the ability to initiate biocatalytic cascades under special conditions holds tremendous potential for therapeutic applications. Herein, conformationally gated nanoreactors that respond to the acidic microenvironment of infected diabetic wounds… read more here.
Keywords: diabetic chronic; chronic wound; microenvironment; cascade reactions ... See more keywords
Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-024-84624-5
Abstract: Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD),… read more here.
Keywords: hydroxytyrosol; hydroxytyrosol via; cascade synthesis; synthesis hydroxytyrosol ... See more keywords
Covalently assembled ultrathin polymer nanocapsules to mimic a multienzyme-cascade antioxidative system
Sign Up to like & getrecommendations! Published in 2020 at "Materials Chemistry Frontiers"
DOI: 10.1039/d0qm00389a
Abstract: Ultrathin selenium-containing manganese-porphyrin-based polymer nanocapsules with both superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity were successfully constructed through a covalent assembly strategy for mimicking the natural multienzyme-cascade antioxidative system. In this case, Mn-CATPyP (4,4′,4′′,4′′′-(Mn-porphyrin-5,10,15,20-tetrayl)tetrakis… read more here.
Keywords: multienzyme cascade; antioxidative system; polymer; cascade antioxidative ... See more keywords
Constructing a multienzyme cascade redox-neutral system for the synthesis of halogenated indoles.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc00811d
Abstract: Inspired by biocatalytic retrosynthesis, a multienzyme cascade system containing alcohol dehydrogenase, flavin-dependent halogenase and flavin reductase was developed for the synthesis of several halogenated indoles starting from amino alcohol. This redox-neutral system not only omitted… read more here.
Keywords: system; neutral system; redox neutral; multienzyme cascade ... See more keywords
Construction of a multienzyme cascade reaction system and its application in D-tagatose biosynthesis
Sign Up to like & getrecommendations! Published in 2025 at "AMB Express"
DOI: 10.1186/s13568-025-01830-8
Abstract: D-tagatose, a low-calorie rare sugar, has significant potential in food, medicine, cosmetics, and other industries owing to its high application value and market potential. In this study, Escherichia coli BL21 was used as the starting… read more here.
Keywords: application; tagatose biosynthesis; system; multienzyme cascade ... See more keywords