Efficient strategy for introducing large and multiple changes in plasmid DNA
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DOI: 10.1038/s41598-018-20169-8
Abstract: While the QuikChange site-directed mutagenesis method and its later modifications are extremely useful and simple, they suffer from several drawbacks. Here, we propose a new method, named LFEAP mutagenesis (Ligation of Fragment Ends After PCR)… read more here.
Keywords: introducing large; multiple changes; dna; large multiple ... See more keywords