Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification
Sign Up to like & getrecommendations! Published in 2021 at "RSC Advances"
DOI: 10.1039/d0ra09021b
Abstract: DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used… read more here.
Keywords: dna; real time; amplification; multiple displacement ... See more keywords
Multiple displacement amplification as the first step can increase the diagnostic efficiency of preimplantation genetic testing for monogenic disease for β‐thalassemia
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Obstetrics and Gynaecology Research"
DOI: 10.1111/jog.14003
Abstract: To evaluate whether using multiple displacement amplification (MDA) as the first step can increase the diagnostic efficiency of preimplantation genetic testing for monogenic disease (PGT‐M) for β‐thalassemia. read more here.
Keywords: diagnostic efficiency; displacement amplification; multiple displacement; step increase ... See more keywords
Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification
Sign Up to like & getrecommendations! Published in 2019 at "BioMed Research International"
DOI: 10.1155/2019/2756516
Abstract: Circular RNAs (circRNAs) are newly discovered incipient non-coding RNAs with potential roles in disease progression in living organisms. Significant reports, since their inception, highlight the abundance and putative functional roles of circRNAs in every organism… read more here.
Keywords: template dependent; illumina sequencing; dependent multiple; displacement amplification ... See more keywords
Template-dependent multiple displacement amplification for profiling human circulating RNA.
Sign Up to like & getrecommendations! Published in 2017 at "BioTechniques"
DOI: 10.2144/000114566
Abstract: Multiple displacement amplification (MDA) is widely used in whole-genome/transcriptome amplification. However, template-independent amplification (TIA) in MDA is a commonly observed phenomenon, particularly when using high concentrations of random hexamer primers and extended incubation times. Here,… read more here.
Keywords: template dependent; circulating rna; displacement amplification; multiple displacement ... See more keywords
Back to Basics: A Simplified Improvement to Multiple Displacement Amplification for Microbial Single-Cell Genomics
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24054270
Abstract: Microbial single-cell genomics (SCG) provides access to the genomes of rare and uncultured microorganisms and is a complementary method to metagenomics. Due to the femtogram-levels of DNA in a single microbial cell, sequencing the genome… read more here.
Keywords: cell; multiple displacement; amplification; single cell ... See more keywords
Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Limited DNA Sequencing Based on Tube and Droplet
Sign Up to like & getrecommendations! Published in 2020 at "Micromachines"
DOI: 10.3390/mi11070645
Abstract: Whole genome amplification (WGA) is crucial for whole genome sequencing to investigate complex genomic alteration at the single-cell or even single-molecule level. Multiple displacement amplification (MDA) and multiple annealing and looping based amplification cycles (MALBAC)… read more here.
Keywords: mda; displacement amplification; amplification mda; multiple displacement ... See more keywords