Multiple gene expression in plants using MIDAS‐P, a versatile type II restriction‐based modular expression vector
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28073
Abstract: MIDAS‐P is a plant expression vector with blue/white screening for iterative cloning of multiple, tandemly arranged transcription units (TUs). We have used the MIDAS‐P system to investigate the expression of up to five genes encoding… read more here.
Keywords: expression vector; multiple gene; anti hiv; expression ... See more keywords
Establishment of a markerless multiple‐gene deletion method based on Cre/loxP mutant system for Bacillus pumilus
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201700370
Abstract: In this study, we established a Cre/loxP mutant recombination system (Cre/lox71‐66 system) for markerless gene deletion to facilitate our follow‐up rational genetic engineering to the strain Bacillus pumilus W3. This modified method uses two mutant… read more here.
Keywords: system; loxp; cre; method ... See more keywords
RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00628
Abstract: Efficient and versatile DNA assembly frameworks have had an impact on promoting synthetic biology to build complex biological systems. To accelerate system development, laboratory automation (or biofoundry) provides an opportunity to construct organisms and DNA… read more here.
Keywords: robotics; multiple gene; robomoclo; gene ... See more keywords
Forced Recycling of an AMA1-Based Genome-Editing Plasmid Allows for Efficient Multiple Gene Deletion/Integration in the Industrial Filamentous Fungus Aspergillus oryzae
Sign Up to like & getrecommendations! Published in 2018 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01896-18
Abstract: Multiple gene modifications of specific fungal strains are required for achieving industrial-scale production of enzymes and secondary metabolites. In the present study, we developed an efficient multiple genetic engineering technique for the filamentous fungus Aspergillus… read more here.
Keywords: genome editing; multiple gene; genetic engineering; gene ... See more keywords