A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-00654-2
Abstract: The most powerful way to probe protein function is to characterize the consequence of its deletion. Compared to conventional gene knockout (KO), conditional knockout (cKO) provides an advanced gene targeting strategy with which gene deletion… read more here.
Keywords: targeting multiple; multiple genes; strategy; conditional knockout ... See more keywords
Multiple genes surrounding Bcl-xL, a common retroviral insertion site, can influence hematopoiesis individually or in concert.
Sign Up to like & getrecommendations! Published in 2020 at "Human gene therapy"
DOI: 10.1089/hum.2019.344
Abstract: Retroviral insertional mutagenesis (RIM) is both a relevant risk in gene therapy and a powerful tool for identifying genes that enhance the competitiveness of repopulating hematopoietic stem and progenitor cells (HSPCs). However, focusing only on… read more here.
Keywords: insertion; insertion site; bcl; gene ... See more keywords
Protein and RNA quantification of multiple genes in single cells.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2018-0130
Abstract: Single-cell analysis overcomes the problems of cellular heterogeneity by revealing the individual differences between cells in tissue. The current tools used to profile gene expression at the single-cell level are arduous and often require specialized… read more here.
Keywords: single cell; rna quantification; expression; protein rna ... See more keywords