Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Journal"
DOI: 10.1042/bcj20210565
Abstract: Plant and fungal THI4 thiazole synthases produce the thiamin thiazole moiety in aerobic conditions via a single-turnover suicide reaction that uses an active-site Cys residue as sulfur donor. Multiple-turnover (i.e. catalytic) THI4s lacking an active-site… read more here.
Keywords: turnover; thi4s; thi4 thiazole; cys ... See more keywords
DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad233
Abstract: Abstract The RNA-guided Cas9 endonuclease from Staphylococcus aureus (SauCas9) can catalyze multiple-turnover reactions whereas Cas9 from Streptococcus pyogenes (SpyCas9) is a single-turnover enzyme. Here we dissect the mechanism of multiple-turnover catalysis by SauCas9 and elucidate… read more here.
Keywords: multiple turnover; product; turnover; dna rehybridization ... See more keywords