Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of fluorescence"
DOI: 10.1007/s10895-021-02876-0
Abstract: This study was to develop a multiplex fluorescent PCR for Shigella detection and species identification. Five primer pairs for Shigella detection and species identification were designed by Primer Premier 5.0. The multiplex fluorescent PCR was… read more here.
Keywords: multiplex fluorescent; fluorescent pcr; detection species; shigella detection ... See more keywords
Cyclic multiplex fluorescent immunohistochemistry and machine learning reveal distinct states of astrocytes and microglia in normal aging and Alzheimer’s disease
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Neuroinflammation"
DOI: 10.1186/s12974-022-02383-4
Abstract: Background Astrocytes and microglia react to Aβ plaques, neurofibrillary tangles, and neurodegeneration in the Alzheimer’s disease (AD) brain. Single-nuclei and single-cell RNA-seq have revealed multiple states or subpopulations of these glial cells but lack spatial… read more here.
Keywords: fluorescent immunohistochemistry; machine learning; multiplex fluorescent; astrocytes microglia ... See more keywords
Development of a visual multiplex fluorescent LAMP assay for the detection of foot-and-mouth disease, vesicular stomatitis and bluetongue viruses
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DOI: 10.1371/journal.pone.0278451
Abstract: Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique that can be used to amplify target genes at a constant temperature, and it has several advantages, including convenience, specificity and sensitivity. However, due to… read more here.
Keywords: lamp; visual multiplex; detection; multiplex fluorescent ... See more keywords
Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens
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DOI: 10.1371/journal.pone.0328651
Abstract: Background Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are common fastidious bacteria responsible for respiratory tract infections, particularly in children and immunocompromised individuals. Due to their demanding growth requirements, traditional culture methods often yield low… read more here.
Keywords: detection; multiplex fluorescent; culture; fluorescent pcr ... See more keywords