A Versatile Nonviral Delivery System for Multiplex Gene-Editing in the Liver.
Sign Up to like & getrecommendations! Published in 2020 at "Advanced materials"
DOI: 10.1002/adma.202003537
Abstract: Recent advances in CRISPR present attractive genome-editing toolsets for therapeutic strategies at the genetic level. Here, a liposome-coated mesoporous silica nanoparticle (lipoMSN) is reported as an effective CRISPR delivery system for multiplex gene-editing in the… read more here.
Keywords: gene editing; multiplex gene; delivery system; editing liver ... See more keywords
Crohn's Colitis: Development of a multiplex gene expression assay comparing mRNA levels of susceptibility genes.
Sign Up to like & getrecommendations! Published in 2017 at "Clinics and research in hepatology and gastroenterology"
DOI: 10.1016/j.clinre.2017.02.004
Abstract: Crohn's disease (CD) is a multifactorial immunologically mediated disease. In this study we explored, for the first time, the efficacy of the Multiplex Gene Assay technology for detecting mRNA expression profile of 24 selected CD… read more here.
Keywords: multiplex gene; response; expression genes; expression ... See more keywords
Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors
Sign Up to like & getrecommendations! Published in 2017 at "Nature methods"
DOI: 10.1038/nmeth.4483
Abstract: Targeted and inducible regulation of mammalian gene expression is a broadly important capability. We engineered drug-inducible catalytically inactive Cpf1 nuclease fused to transcriptional activation domains to tune the expression of endogenous genes in human cells.… read more here.
Keywords: gene regulation; multiplex gene; cpf1; gene ... See more keywords
Efficient multitool/multiplex gene engineering with TALE-BE
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.1033669
Abstract: TALE base editors are a recent addition to the genome editing toolbox. These molecular tools are fusions of a transcription activator-like effector domain (TALE), split-DddA deaminase halves, and an uracil glycosylase inhibitor (UGI) that have… read more here.
Keywords: knock; efficient multitool; multiplex gene; multitool multiplex ... See more keywords