Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1076
Abstract: Abstract Recombineering assisted multiplex genome editing generally uses single-stranded oligonucleotides for site directed mutational changes. It has proven highly efficient for functional screens and to optimize microbial cell factories. However, this approach is limited to… read more here.
Keywords: improved dsdna; multiplex genome; genome; genome engineering ... See more keywords
Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology for Biofuels"
DOI: 10.1186/s13068-019-1537-1
Abstract: Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the… read more here.
Keywords: genome editing; system; efficiency; crispr cas9n ... See more keywords