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   Articles with "multiplex genome" as a keyword



ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism

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recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-54191-4

Abstract: The complexities encountered in microbial metabolic engineering continue to elude prediction and design. Unravelling these complexities requires strategies that go beyond conventional genetics. Using multiplex mutagenesis with double stranded (ds) DNA, we extend the multiplex… read more here.

Keywords: multiplex genome; real mge; engineering; malonyl coa ... See more keywords

Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria

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recommendations! Published in 2021 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkab1076

Abstract: Abstract Recombineering assisted multiplex genome editing generally uses single-stranded oligonucleotides for site directed mutational changes. It has proven highly efficient for functional screens and to optimize microbial cell factories. However, this approach is limited to… read more here.

Keywords: improved dsdna; multiplex genome; genome; genome engineering ... See more keywords

Directional improvement of agronomic traits in salt-tolerant rice by multiplex-genome-editing.

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recommendations! Published in 2025 at "Journal of integrative plant biology"

DOI: 10.1111/jipb.13926

Abstract: Soil salinization has emerged as a major threat affecting crop yields. Global warming leads to a massive loss of terrestrial water and makes soils saltier. Cultivating salt-tolerant crops is the major strategy adopted for utilizing… read more here.

Keywords: rice; tolerant rice; salt tolerant; agronomic traits ... See more keywords

Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis

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recommendations! Published in 2019 at "Biotechnology for Biofuels"

DOI: 10.1186/s13068-019-1537-1

Abstract: Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the… read more here.

Keywords: genome editing; system; efficiency; crispr cas9n ... See more keywords