Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1205-7_40
Abstract: The presence of tertiary lymphoid structures (TLS) is correlated with prolonged patient survival in a variety of solid cancers, including melanoma. However, few methods have been described that could enable a more comprehensive understanding of… read more here.
Keywords: immune; infiltrates melanoma; multiplex immunofluorescence; lymphoid structures ... See more keywords
Multiplex Immunofluorescence Assays.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9773-2_22
Abstract: Multiplexed imaging platforms to simultaneously detect multiple epitopes in the same tissue section emerged in the last years as very powerful tools to study tumor immune contexture. These revolutionary technologies are providing a deep methodology… read more here.
Keywords: immunofluorescence assays; multiplex immunofluorescence; tissue; multiplexed imaging ... See more keywords
Automated quantitative high-throughput multiplex immunofluorescence pipeline to evaluate OXPHOS defects in formalin-fixed human prostate tissue
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DOI: 10.1038/s41598-022-10588-z
Abstract: Advances in multiplex immunofluorescence (mIF) and digital image analysis has enabled simultaneous assessment of protein defects in electron transport chain components. However, current manual methodology is time consuming and labour intensive. Therefore, we developed an… read more here.
Keywords: methodology; prostate; oxphos; multiplex immunofluorescence ... See more keywords
BIOM-27. PREDICTIVE EVALUATION OF QUANTITATIVE SPATIAL PROFILING OF THE TUMOR MICROENVIRONMENT BY MULTIPLEX IMMUNOFLUORESCENCE IN RECURRENT GLIOBLASTOMA TREATED WITH PD-1 INHIBITORS
Sign Up to like & getrecommendations! Published in 2020 at "Neuro-oncology"
DOI: 10.1093/neuonc/noaa215.027
Abstract: PD-1 inhibitors have shown limited efficacy in glioblastoma (GBM) due to microenvironment immunosuppression and low tumor mutational burden. In GBM, PD-L1 expression is not a predictive marker for response to PD-1 or PD-L1 inhibitors. Multiplex… read more here.
Keywords: quantitative spatial; glioblastoma; multiplex immunofluorescence; tumor microenvironment ... See more keywords
In situ multiplex immunofluorescence analysis of the inflammatory burden in kidney allograft rejection: A new tool to characterize the alloimmune response
Sign Up to like & getrecommendations! Published in 2019 at "American Journal of Transplantation"
DOI: 10.1111/ajt.15699
Abstract: The exact composition of leukocyte infiltration during kidney allograft rejection is difficult to comprehend and visualize on the same biopsy slide. Using an innovative technology of multiplex immunofluorescence (mIF), we were able to detect simultaneously… read more here.
Keywords: multiplex immunofluorescence; allograft rejection; rejection; kidney allograft ... See more keywords
Utilisation of cytological samples for multiplex immunofluorescence assay
Sign Up to like & getrecommendations! Published in 2021 at "Cytopathology"
DOI: 10.1111/cyt.12979
Abstract: Understanding the immune environment of non‐small cell lung cancer (NSCLC) is important for designing effective anticancer immunotherapies. We describe the use of multiplex immunofluorescence (mIF) assays to enable characterisation of the tumour‐infiltrating immune cells and… read more here.
Keywords: utilisation cytological; multiplex immunofluorescence; cytological samples; immunofluorescence ... See more keywords
Abstract 2950: Biomarkers of immune infiltration for multiplex immunofluorescence in breast cancer
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Research"
DOI: 10.1158/1538-7445.am2017-2950
Abstract: Introduction: Only a subset of patients have a clinical response to cancer immunotherapy. The molecular mechanisms that mediate the immunological response or tolerance to treatment are just beginning to be understood. Several tumor signaling pathways… read more here.
Keywords: cancer; multiplex immunofluorescence; immune infiltration; breast cancer ... See more keywords
Abstract 4148: Immunophenotyping of responses to immunotherapy in syngeneic tumor models using multiplex immunofluorescence compared to multiparametric flow cytometry
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-4148
Abstract: In the last decades, several major breakthroughs in cancer treatment were achieved through a better understanding and therapeutic targeting of the immune system. Multiparametric flow cytometry is a robust and powerful tool to evaluate the… read more here.
Keywords: flow cytometry; tumor models; multiplex immunofluorescence; immune ... See more keywords
Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24043086
Abstract: Traditional immunohistochemistry (IHC) has already become an essential method of diagnosis and therapy in cancer management. However, this antibody-based technique is limited to detecting a single marker per tissue section. Since immunotherapy has revolutionized the… read more here.
Keywords: immunohistochemistry; immunotherapy; multiplex immunofluorescence; cancer ... See more keywords