Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22230
Abstract: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT‐PCR assays for RV. The… read more here.
Keywords: detection respiratory; respiratory viruses; pcr assays; multiplex pcr ... See more keywords
Developing a multiplex PCR‐based assay kit for bloodstream infection by analyzing genomic big data
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24686
Abstract: In recent years, the incidence of bloodstream infections (BSI) has increased, the composition of pathogenic bacteria has changed, and drug resistance among bacteria has gradually increased due to the widespread use of interventional techniques, broad‐spectrum… read more here.
Keywords: bloodstream; assay kit; developing multiplex; pcr based ... See more keywords
A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8837-2_11
Abstract: Polymerase chain reaction (PCR) methods using phytoplasma-specific primers are widely used to detect phytoplasmas from infected plants and insects. Here, I describe a method of multiplex-PCR to amplify nine gene fragments in PCR reactions from… read more here.
Keywords: method; pcr; group phytoplasmas; multiplex pcr ... See more keywords
Development of multiplex PCR assay for detection of Alternaria brassicae, A. brassicicola and Xanthomonas campestris pv. campestris in crucifers.
Sign Up to like & getrecommendations! Published in 2022 at "Archives of microbiology"
DOI: 10.1007/s00203-022-02846-5
Abstract: Among biotic stresses, Alternaria leaf spots caused by Alternaria brassicae and A. brassicicola and black rot caused by Xanthomonas campestris pv. campestris are major limiting factors in brassica cultivation across the world. Because of seed-borne… read more here.
Keywords: campestris campestris; brassicae brassicicola; multiplex pcr; seed ... See more keywords
Multiplex PCR Unyvero i60 ITI application improves detection of low-virulent microorganisms in periprosthetic joint infections
Sign Up to like & getrecommendations! Published in 2018 at "International Orthopaedics"
DOI: 10.1007/s00264-018-4136-z
Abstract: PurposeThe aim of this study was to evaluate the pre-operative performance of an automated multiplex PCR (mPCR) system in patients with suspected periprosthetic joint infection (PJI).MethodsUnder sterile conditions, synovial fluid samples from patients with a… read more here.
Keywords: mpcr; detection; synovial fluid; multiplex pcr ... See more keywords
Comparative Study of GeneXpert MTB/RIF Assay and Multiplex PCR Assay for Direct Detection of Mycobacterium tuberculosis in Suspected Pulmonary Tuberculosis Patients
Sign Up to like & getrecommendations! Published in 2017 at "Current Microbiology"
DOI: 10.1007/s00284-017-1279-x
Abstract: Pulmonary tuberculosis (PTB) is one of the major infectious diseases in developing countries. The objective of this study was to compare rapid diagnostics technique, GeneXpert MTB/RIF (GeneXpert) and Multiplex PCR assay (MPCR) targeting IS6110 segment… read more here.
Keywords: pulmonary tuberculosis; mtb; study; genexpert ... See more keywords
Development of a 16 X-STR multiplex PCR system for kinship analysis and its applicability for the Sinhalese population in Sri Lanka
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-020-02450-6
Abstract: The study describes the development of a new multiplex PCR system that simultaneously amplifies 16 X chromosome short tandem repeats (X-STRs) loci in a single PCR reaction and its applicability on a sample of 200… read more here.
Keywords: pcr; multiplex pcr; pcr system; sinhalese population ... See more keywords
Simultaneous Expression of GUS and Actin Genes by Using the Multiplex RT-PCR and Multiplex Gold Nanoparticle Probes
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Fluorescence"
DOI: 10.1007/s10895-018-2225-4
Abstract: Gene expression analysis is considered to be extremely important in many different biological researches. DNA-based diagnostic test, which contributes to DNA identification, has higher specificity, cost, and speed than some biochemical and molecular methods. In… read more here.
Keywords: pcr multiplex; gene; expression; multiplex pcr ... See more keywords
A pilot study on PCR-based detection of four foodborne pathogenic microorganisms
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Food Measurement and Characterization"
DOI: 10.1007/s11694-017-9680-5
Abstract: To establish PCR-based detection methods for Staphylococcus aureus, Shigella, Pasteurella multocida and Pseudomonas aeruginosa, the nuc, ipah, ptfa and oprl genes were amplified by singleplex PCRs and multiplex PCR using specific primers that were designed… read more here.
Keywords: detection; singleplex pcrs; pcr; based detection ... See more keywords
Multiplex PCR with the blunt hairpin primers for next generation sequencing
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-017-0133-0
Abstract: The multiplex PCR is one of the important methods to enrich the target DNAs for next generation sequencing. The non-specific amplification and interaction between the primers are the pivotal challenges of multiplex PCR. Here, we… read more here.
Keywords: blunt hairpin; hairpin primers; hairpin; multiplex pcr ... See more keywords
Simple and fast multiplex PCR method for detection of species origin in meat products
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-017-2980-2
Abstract: Identification of animal species is one of the major concerns in food regulatory control and quality assurance system. Different approaches have been used for species identification in animal origin of feedstuff. This study aimed to… read more here.
Keywords: origin meat; method; pcr; meat ... See more keywords