A novel multiplex qPCR method for assessing the comparative lengths of telomeres
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.23929
Abstract: The comparative length of telomeres is considered to be related to diseases such as cancer, aging, and cardiovascular diseases. qPCR is currently one of the main methods for detecting telomere length. However, due to the… read more here.
Keywords: assessing comparative; qpcr method; method assessing; comparative lengths ... See more keywords
A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Reports"
DOI: 10.1016/j.btre.2020.e00447
Abstract: Highlights • Quantitative polymerase chain reactions specifically detect Trichoderma atroviride.• The method uses a probe based on the tef-1α for the detection.• The method simultaneously detects T. atroviride and Botrytis cinerea in blackberries. read more here.
Keywords: botrytis cinerea; qpcr taqman; trichoderma atroviride; multiplex qpcr ... See more keywords
Corrigendum to ‘A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections’
Sign Up to like & getrecommendations! Published in 2021 at "EBioMedicine"
DOI: 10.1016/j.ebiom.2021.103261
Abstract: a Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, United Kingdom b PHE Malaria Reference Laboratory, London School of Hygiene & Tropical Medicine, United Kingdom c Centre of Statistics and Applications of… read more here.
Keywords: medicine; united kingdom; corrigendum novel; multiplex qpcr ... See more keywords
New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time.
Sign Up to like & getrecommendations! Published in 2018 at "Leukemia research"
DOI: 10.1016/j.leukres.2018.04.001
Abstract: Fast identification of BCR-ABL fusion genes is critical for precise diagnosis, risk stratification and therapy scheme selection in leukemia. More convenient methods are needed for quickly detection of the BCR-ABL fusion genes. Multiplex fluorescent reverse… read more here.
Keywords: fusion genes; bcr; abl fusion; bcr abl ... See more keywords
Rapid and accurate diagnosis of Chlamydia trachomatis in the urogenital tract by a dual-gene multiplex qPCR method.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of medical microbiology"
DOI: 10.1099/jmm.0.001084
Abstract: Introduction. Chlamydia trachomatis (C. trachomatis, CT) is an obligatory intracellular bacterium that causes urogenital tract infections and leads to severe reproductive consequences. Therefore, a rapid and accurate detection method with high sensitivity and specificity is… read more here.
Keywords: qpcr method; method; microbiology; multiplex qpcr ... See more keywords
Multiplex qPCR assay to determine Leishmania infantum load in Lutzomyia longipalpis sandfly samples.
Sign Up to like & getrecommendations! Published in 2022 at "Medical and veterinary entomology"
DOI: 10.1111/mve.12564
Abstract: The study aimed to develop a multiplex qPCR to detect Leishmania infantum load in different sandfly sample settings using Leishmania kDNA and sandfly vacuolar ATPase (VATP) subunit C as internal control gene. The amplification of… read more here.
Keywords: sandfly; multiplex qpcr; infantum; leishmania infantum ... See more keywords
Optimization of multiplex quantitative polymerase chain reaction based on response surface methodology and an artificial neural network-genetic algorithm approach
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0200962
Abstract: Multiplex quantitative polymerase chain reaction (qPCR) has found an increasing range of applications. The construction of a reliable and dynamic mathematical model for multiplex qPCR that analyzes the effects of interactions between variables is therefore… read more here.
Keywords: multiplex qpcr; methodology; qpcr; multiplex quantitative ... See more keywords
Optimizing, validating, and field testing a multiplex qPCR for the detection of amphibian pathogens.
Sign Up to like & getrecommendations! Published in 2018 at "Diseases of aquatic organisms"
DOI: 10.3354/dao03230
Abstract: Amphibian populations worldwide are facing numerous threats, including the emergence and spread of infectious diseases. In the past 2 decades, Batrachochytrium dendrobatidis (Bd), a parasitic fungus, and a group of viruses comprising the genus Ranavirus… read more here.
Keywords: multiplex qpcr; detection; field testing; validating field ... See more keywords
Development of a multiplex qPCR for the quantification of three protozoan parasites of the eastern oyster Crassostrea virginica.
Sign Up to like & getrecommendations! Published in 2022 at "Diseases of aquatic organisms"
DOI: 10.3354/dao03694
Abstract: A multiplex quantitative PCR (qPCR) assay for the simultaneous detection of 3 eastern oyster Crassostrea virginica parasites, Perkinsus marinus, Haplosporidium nelsoni, and H. costale, was developed using 3 different fluorescently labeled hydrolysis probes. The primers… read more here.
Keywords: qpcr; oyster crassostrea; crassostrea virginica; multiplex qpcr ... See more keywords