Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two‐tube multiplex real‐time RT‐PCR assay
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.27658
Abstract: Due to the concurrent prevalence and increasing risk of coinfection of the clinically important Arboviruses, timely and accurate differential diagnosis is important for clinical management and the epidemiological investigation. A two‐tube multiplex real‐time reverse transcription‐polymerase… read more here.
Keywords: tube multiplex; detection; pcr assay; two tube ... See more keywords
Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7231-9_15
Abstract: Multiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has drawn unprecedented attention as a means of verifying the rapidly increasing genetic targets in a single phenotype. We report the detailed… read more here.
Keywords: multiplex real; real time; time pcr; pcr ... See more keywords
Multiplex real-time PCR for the detection of insect DNA and determination of contents of Tenebrio molitor, Locusta migratoria and Achaeta domestica in food
Sign Up to like & getrecommendations! Published in 2019 at "European Food Research and Technology"
DOI: 10.1007/s00217-018-03225-5
Abstract: In Europe, edible insects are not a part of a common diet. But according to the Food and Agriculture Organization of the united nations (FAO), animal protein from insects could be an ecological, economic and… read more here.
Keywords: insects; multiplex real; real time; time pcr ... See more keywords
Multiplex real-time PCR for the detection and quantification of DNA from chamois, roe, deer, pork and beef
Sign Up to like & getrecommendations! Published in 2020 at "European Food Research and Technology"
DOI: 10.1007/s00217-020-03468-1
Abstract: Meat originating from roe, deer and especially chamois is expensive. Normally, such delicacies are provided directly by hunters and exclusively during the hunting season. In some cases, either to achieve higher profit or to enable… read more here.
Keywords: multiplex real; pork beef; real time; time pcr ... See more keywords
A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09835-7
Abstract: Swine enteric coronaviruses are a group of most significant pathogens causing diarrhea in piglets with similar clinical symptoms and pathological changes. To develop a simple, rapid, accurate, and high-throughput detection method for diagnosis and differential… read more here.
Keywords: multiplex real; detection; real time; time qpcr ... See more keywords
Development of a multiplex real-time PCR surveillance assay for monitoring the health status of Ecuadorian amphibians at risk of extinction
Sign Up to like & getrecommendations! Published in 2019 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2019.10.134
Abstract: Abstract Chytrid fungi and viruses within the genus Ranavirus have been associated with mass mortality events and declines in amphibian populations worldwide. The fungus Batrachochytrium dendrobatidis (Bd) was reported in Ecuador; however, other chytrid fungi… read more here.
Keywords: multiplex real; risk extinction; development multiplex; surveillance ... See more keywords
A multiplex real-time RT-PCR for simultaneous detection of four most common avian respiratory viruses.
Sign Up to like & getrecommendations! Published in 2018 at "Virology"
DOI: 10.1016/j.virol.2017.11.021
Abstract: A one-step multiplex real-time reverse transcription-PCR (rRT-PCR) assay was developed for simultaneous detection and quantification of four avian respiratory viruses: avian influenza virus (AIV), infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and infectious laryngotracheitis… read more here.
Keywords: multiplex real; detection; real time; pcr ... See more keywords
Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe
Sign Up to like & getrecommendations! Published in 2022 at "ACS Sensors"
DOI: 10.1021/acssensors.1c02079
Abstract: Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting the efficiency of primer binding. Such variants that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them… read more here.
Keywords: hfman probe; probe; sars cov; real time ... See more keywords
Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With Euwallacea kuroshio and Euwallacea whitfordiodendrus in California.
Sign Up to like & getrecommendations! Published in 2019 at "Plant disease"
DOI: 10.1094/pdis-01-19-0201-re
Abstract: California has been invaded by two distinct Euwallacea spp. that vector unique plant pathogenic symbiotic fungi on multiple hosts and cause Fusarium dieback. The objective of this study was to develop multiplex real-time quantitative PCR… read more here.
Keywords: euwallacea whitfordiodendrus; time; real time; euwallacea kuroshio ... See more keywords
Enhanced detection and serotyping of foot‐and‐mouth disease virus serotype O, A, and Asia1 using a novel multiplex real‐time RT‐PCR
Sign Up to like & getrecommendations! Published in 2022 at "Transboundary and Emerging Diseases"
DOI: 10.1111/tbed.14603
Abstract: Abstract Rapid and accurate detection and serotyping of foot‐and‐mouth disease (FMD) virus (FMDV) is essential for implementing control policies against emergent FMD outbreaks. Current serotyping assays, such as VP1 reverse transcription‐polymerase chain reaction (RT‐PCR)/sequencing (VP1… read more here.
Keywords: time pcr; detection; real time; pcr ... See more keywords
Paradigm for diagnosing mycobacterial disease: direct detection and differentiation of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in clinical specimens using multiplex real-time PCR
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Pathology"
DOI: 10.1136/jclinpath-2017-204945
Abstract: Aims Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) are clinically different, and the rapid detection and differentiation of M. tuberculosis complex (MTBC) and NTM is crucial for patient management and infection control. Given the slow growth… read more here.
Keywords: multiplex real; real time; time pcr; clinical specimens ... See more keywords