Multiplex Immunofluorescence Assays.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9773-2_22
Abstract: Multiplexed imaging platforms to simultaneously detect multiple epitopes in the same tissue section emerged in the last years as very powerful tools to study tumor immune contexture. These revolutionary technologies are providing a deep methodology… read more here.
Keywords: immunofluorescence assays; multiplex immunofluorescence; tissue; multiplexed imaging ... See more keywords
Pattern Recognition of Cells via Multiplexed Imaging with Monosaccharide-Imprinted Quantum Dots.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00965
Abstract: Recognition of cancer cells is essential for many important areas such as targeted cancer therapy. Multimonosaccharide-based recognition could be a useful strategy to improve the recognition specificity, but such a possibility has not been explored… read more here.
Keywords: pattern recognition; monosaccharide imprinted; recognition; cancer ... See more keywords
Fluorescent biosensors for multiplexed imaging of phosphoinositide dynamics.
Sign Up to like & getrecommendations! Published in 2019 at "ACS chemical biology"
DOI: 10.1021/acschembio.9b00691
Abstract: Phosphoinositides constitute a critical family of lipids that regulate numerous cellular processes. Phosphatidylinositol 4,5-bisphosphate (PIP2) is arguably the most important plasma membrane phosphoinositide and is involved in regulating diverse processes. It is also the precursor… read more here.
Keywords: fluorescent; imaging phosphoinositide; fluorescent biosensors; multiplexed imaging ... See more keywords
Deep cell phenotyping and spatial analysis of multiplexed imaging with TRACERx-PHLEX
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-48870-5
Abstract: The growing scale and dimensionality of multiplexed imaging require reproducible and comprehensive yet user-friendly computational pipelines. TRACERx-PHLEX performs deep learning-based cell segmentation (deep-imcyto), automated cell-type annotation (TYPEx) and interpretable spatial analysis (Spatial-PHLEX) as three independent… read more here.
Keywords: multiplexed imaging; analysis; tracerx phlex; cell ... See more keywords
Tribus: semi-automated discovery of cell identities and phenotypes from multiplexed imaging and proteomic data
Sign Up to like & getrecommendations! Published in 2024 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btaf082
Abstract: Motivation Multiplexed imaging and single-cell analysis are increasingly applied to investigate the tissue spatial ecosystems in cancer and other complex diseases. Accurate single-cell phenotyping based on marker combinations is a critical but challenging task due… read more here.
Keywords: multiplexed imaging; automated discovery; cell; tribus ... See more keywords
Multiplexed imaging mass cytometry elicits p-S6 as a therapeutic and prognostic factor in diffuse large B-cell lymphoma
Sign Up to like & getrecommendations! Published in 2025 at "Blood Advances"
DOI: 10.1182/bloodadvances.2024015469
Abstract: Key Points • p-S6 is particularly overexpressed in Myc/Bcl-2 DE DLBCL, indicating poor prognosis of patients with DLBCL.• The inhibition of p-S6 in fibroblastic cells suppressed DLBCL cell proliferation and exhibited synergistic effects with chemotherapy. read more here.
Keywords: multiplexed imaging; mass cytometry; cell; elicits therapeutic ... See more keywords