Improvement of R-2-(4-hydroxyphenoxy)propionic acid biosynthesis of Beauveria bassiana by combined mutagenesis.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.1872
Abstract: R-2-(4-hydroxyphenoxy)propionicacid (HPOPA) is a valuable intermediate for the synthesis of enantiomerically pure aryloxyphenoxypropionic acid herbicides. In this work, to improve the HPOPA biosynthesis by Beauveria bassiana ZJB16002 from the substrate R-2-phenoxypropionic acid (POPA), the original… read more here.
Keywords: mutagenesis; hpopa; beauveria bassiana; acid ... See more keywords
Casting epPCR (cepPCR): A simple random mutagenesis method to generate high quality mutant libraries
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26327
Abstract: During the last decade, directed evolution has become a standard protein engineering strategy to reengineer proteins for industrial applications under high stress conditions (e.g., high temperature, extreme pH, ionic liquids, or organic solvents). The most… read more here.
Keywords: mutagenesis; beneficial positions; random mutagenesis; ceppcr ... See more keywords
Combinatorial InVitroFlow-assisted Mutagenesis (CombIMut) yields a 41-fold improved CelA2 cellulase.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28110
Abstract: The combination of diversity generation methods and ultrahigh-throughput screening (uHTS) technologies is key to efficiently explore nature's sequence space and elucidate structure-function relationships of enzymes. Beneficial substitutions often cluster in a few regions and simultaneous… read more here.
Keywords: invitroflow assisted; cela2 cellulase; cela2; mutagenesis ... See more keywords
Stabilization of the Reductase Domain in the Catalytically Self‐Sufficient Cytochrome P450BM3 by Consensus‐Guided Mutagenesis
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201700546
Abstract: The multidomain, catalytically self‐sufficient cytochrome P450 BM‐3 from Bacillus megaterium (P450BM3) constitutes a versatile enzyme for the oxyfunctionalization of organic molecules and natural products. However, the limited stability of the diflavin reductase domain limits the… read more here.
Keywords: p450bm3; mutagenesis; reductase; consensus ... See more keywords
Co‐translational Installation of Posttranslational Modifications by Non‐canonical Amino Acid Mutagenesis
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202300039
Abstract: Protein posttranslational modifications (PTMs) play critical roles in regulating cellular activities. Here we provide a survey of genetic code expansion (GCE) methods that were applied in the co‐translational installation and studies of PTMs through noncanonical… read more here.
Keywords: mutagenesis; amino acid; translational installation; posttranslational modifications ... See more keywords
Lack of hydroxyurea‐associated mutagenesis in pediatric sickle cell disease patients
Sign Up to like & getrecommendations! Published in 2023 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22536
Abstract: Hydroxyurea is approved for treating children and adults with sickle cell anemia (SCA). Despite its proven efficacy, concerns remain about its mutagenic and carcinogenic potential that hamper its widespread use. Cell culture‐ and animal‐based investigations… read more here.
Keywords: cell; sca patients; mutagenesis; mut ret ... See more keywords
The selective breeding and mutagenesis mechanism of high-yielding surfactin Bacillus subtilis strains with atmospheric and room temperature plasma.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.11521
Abstract: BACKGROUND Surfactin, a good biological surfactant, is derived from the metabolites of microorganisms. However, the ability of natural strains to produce surfactin is low, so this study expected to use a novel mutagenesis technology to… read more here.
Keywords: mutagenesis; mutagenesis mechanism; temperature plasma; room temperature ... See more keywords
Enhanced protein glutaminase production from Chryseobacterium proteolyticum combining physico-chemical mutagenesis and resistance screening and its application to soybean protein isolates.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.12535
Abstract: BACKGROUND Protein glutaminase (PG) is a novel protein modification biotechnology that is increasingly being used in the food industry. However, the current level of fermentation of PG-producing strains still does not meet the requirements of… read more here.
Keywords: protein isolate; proteolyticum; protein glutaminase; production ... See more keywords
Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1111-1_8
Abstract: To fully understand the function of cytomegalovirus (CMV) genes, it is imperative that they are studied in the context of infection. Therefore, the targeted deletion of individual viral genes and the comparison of these loss-of-function… read more here.
Keywords: mutagenesis; cytomegalovirus mutants; human cytomegalovirus; construction human ... See more keywords
Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1720-5_3
Abstract: Target deconvolution of new bioactive agents identified from phenotypic screens remains a challenging task. The discovery of mutations that confer resistance to such agents is regarded as the gold standard proof of target identification. Here,… read more here.
Keywords: mutagenesis; crispr; target; essential genes ... See more keywords
All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7577-8_8
Abstract: The technique of all-codon mutagenesis can generate mutants that represent all possible amino acid replacements at any particular residue in a protein. It is thus a powerful tool to probe structure-function relationships in proteins of… read more here.
Keywords: mutagenesis; codon mutagenesis; structure function; mutagenesis structure ... See more keywords