Massive parallel insertion site sequencing of an arrayed Sinorhizobium meliloti signature-tagged mini-Tn 5 transposon mutant library.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2017.02.019
Abstract: Transposon mutagenesis in conjunction with identification of genomic transposon insertion sites is a powerful tool for gene function studies. We have implemented a protocol for parallel determination of transposon insertion sites by Illumina sequencing involving… read more here.
Keywords: mutant library; insertion; transposon; signature tagged ... See more keywords
Deployment of a Vibrio cholerae ordered transposon mutant library in a quorum-competent genetic background
Sign Up to like & getrecommendations! Published in 2025 at "mBio"
DOI: 10.1128/mbio.00036-25
Abstract: ABSTRACT Vibrio cholerae, the causative agent of cholera, has sparked seven pandemics in recent centuries, with the current one being the most prolonged. V. cholerae’s pathogenesis hinges on its ability to switch between low- and… read more here.
Keywords: mutant library; transposon mutant; cholerae; cell ... See more keywords
Identification of Mycobacterium tuberculosis intracellular survival-related virulence factors via CRISPR-based eukaryotic-like secretory protein mutant library screen
Sign Up to like & getrecommendations! Published in 2025 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00767-25
Abstract: ABSTRACT Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), remains a serious infectious disease posing significant global health challenges. A critical evolutionary feature of M.tb is its genome encoding a set of eukaryotic-like secretory proteins, which… read more here.
Keywords: mutant library; intracellular survival; eukaryotic like; secretory proteins ... See more keywords
Robustness quantification of a mutant library screen revealed key genetic markers in yeast
Sign Up to like & getrecommendations! Published in 2024 at "Microbial Cell Factories"
DOI: 10.1186/s12934-024-02490-2
Abstract: Background Microbial robustness is crucial for developing cell factories that maintain consistent performance in a challenging environment such as large-scale bioreactors. Although tools exist to assess and understand robustness at a phenotypic level, the underlying… read more here.
Keywords: mutant library; robustness; library screen; robustness quantification ... See more keywords
CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice
Sign Up to like & getrecommendations! Published in 2024 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2024.1501092
Abstract: The environmental conditions play a crucial role in determining crop yield, which is essential for ensuring food and nutritional security. However, rapid climate change is exacerbating global environmental stress, leading to severe biotic pressures on… read more here.
Keywords: mutant library; library screening; cas9; crispr cas9 ... See more keywords
Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)
Sign Up to like & getrecommendations! Published in 2022 at "Plants"
DOI: 10.3390/plants11050585
Abstract: The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly used background strains of Chlamydomonas are CC-1690 and CC-5325. CC-1690, also called 21gr,… read more here.
Keywords: clip mutant; background; chlamydomonas reinhardtii; mutant library ... See more keywords