High-Throughput Mutant Screening via Transposon Sequencing.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top107867
Abstract: Transposon mutagenesis has been the method of choice for genetic screens and selections in bacteria by virtue of the transposon being linked to the disrupted gene, simplifying its identification. Transposon sequencing (Tn-seq) is a high-throughput… read more here.
Keywords: throughput mutant; high throughput; mutant screening; transposon sequencing ... See more keywords
Fertilization-Defective Gametophytic Mutant Screening: A Novel Approach
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2020.00967
Abstract: Gametophytic mutants share very small proportion of the total mutants generated by any mutagenic approach; even rarer are the fertilization-defective gametophytic mutants. They require an efficient and targeted strategy instead of ‘brute force’ screening approach.… read more here.
Keywords: fertilization; fertilization defective; gametophytic mutants; mutant screening ... See more keywords