Directed Evolution of Proteins Based on Mutational Scanning.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7366-8_6
Abstract: Directed evolution has emerged as one of the most effective protein engineering methods in basic research as well as in applications in synthetic organic chemistry and biotechnology. The successful engineering of protein activity, allostery, binding… read more here.
Keywords: evolution proteins; mutational scanning; chemistry; directed evolution ... See more keywords
Application of Deep Mutational Scanning in Hepatitis C Virus.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8976-8_12
Abstract: Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner.… read more here.
Keywords: deep mutational; mutational scanning; hepatitis virus;
Deep mutational scanning for therapeutic antibody engineering.
Sign Up to like & getrecommendations! Published in 2021 at "Trends in pharmacological sciences"
DOI: 10.1016/j.tips.2021.11.010
Abstract: The biophysical and functional properties of monoclonal antibody (mAb) drug candidates are often improved by protein engineering methods to increase the probability of clinical efficacy. One emerging method is deep mutational scanning (DMS) which combines… read more here.
Keywords: scanning therapeutic; engineering; antibody; mutational scanning ... See more keywords
Elucidating the Determinants of Polymerase Specificity by Microfluidic-Based Deep Mutational Scanning.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00104
Abstract: Engineering polymerases to synthesize artificial genetic polymers with unique backbone structures is limited by a general lack of understanding about the structural determinants that govern substrate specificity. Here, we report a high-throughput microfluidic-based approach for… read more here.
Keywords: deep mutational; microfluidic based; polymerase specificity; mutational scanning ... See more keywords
Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-17081-y
Abstract: RNA-guided endonucleases (RGENs) have invigorated the field of site-specific nucleases. The success of Streptococcus pyogenes Cas9 (SpCas9) has led to the discovery of several other CRISPR-associated RGENs. As more RGENs become available, it will be… read more here.
Keywords: pyogenes cas9; deep mutational; important functional; mutational scanning ... See more keywords
Coordination of -1 programmed ribosomal frameshifting by transcript and nascent chain features revealed by deep mutational scanning
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1172
Abstract: Abstract Programmed ribosomal frameshifting (PRF) is a translational recoding mechanism that enables the synthesis of multiple polypeptides from a single transcript. During translation of the alphavirus structural polyprotein, the efficiency of −1PRF is coordinated by… read more here.
Keywords: ribosomal frameshifting; transcript nascent; chain; mutational scanning ... See more keywords
Deep mutational scanning and machine learning reveal structural and molecular rules governing allosteric hotspots in homologous proteins
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.05.01.490188
Abstract: A fundamental question in protein science is where allosteric hotspots – residues critical for allosteric signaling – are located, and what properties differentiate them. We carried out deep mutational scanning (DMS) of four homologous bacterial… read more here.
Keywords: structural molecular; allosteric hotspots; machine learning; deep mutational ... See more keywords
Mutational scanning identified amino acids of the CLOCK exon 19‐domain essential for circadian rhythms
Sign Up to like & getrecommendations! Published in 2022 at "Acta Physiologica"
DOI: 10.1111/apha.13794
Abstract: In the mammalian circadian clock, the CLOCK/BMAL1 heterodimer binds to E‐box enhancer elements in the promoters of its target genes to activate transcription. The classical Clock mice, the first circadian mouse mutant discovered, are behaviourally… read more here.
Keywords: circadian rhythms; domain; mutational scanning; clock ... See more keywords
DiMSum: an error model and pipeline for analyzing deep mutational scanning data and diagnosing common experimental pathologies
Sign Up to like & getrecommendations! Published in 2020 at "Genome Biology"
DOI: 10.1186/s13059-020-02091-3
Abstract: Deep mutational scanning (DMS) enables multiplexed measurement of the effects of thousands of variants of proteins, RNAs, and regulatory elements. Here, we present a customizable pipeline, DiMSum, that represents an end-to-end solution for obtaining variant… read more here.
Keywords: deep mutational; mutational scanning; error model; pipeline ... See more keywords
Applications of Deep Mutational Scanning in Virology
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13061020
Abstract: Several recently developed high-throughput techniques have changed the field of molecular virology. For example, proteomics studies reveal complete interactomes of a viral protein, genome-wide CRISPR knockout and activation screens probe the importance of every single… read more here.
Keywords: deep mutational; virology; scanning virology; mutational scanning ... See more keywords
Structural and functional characterization of G protein–coupled receptors with deep mutational scanning
Sign Up to like & getrecommendations! Published in 2020 at "eLife"
DOI: 10.7554/elife.54895
Abstract: The >800 human G protein–coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state- and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling… read more here.
Keywords: mutational scanning; protein coupled; coupled receptors; deep mutational ... See more keywords