A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein–protein binding
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DOI: 10.1038/s41598-020-77992-1
Abstract: The reverse two-hybrid system is a powerful method to select mutations that disrupt the interaction between two proteins and therefore to identify the residues involved in this interaction. However, the usefulness of this technique has… read more here.
Keywords: protein; mutations disrupt; two hybrid; missense mutations ... See more keywords
RRM adjacent TARDBP mutations disrupt RNA binding and enhance TDP-43 proteinopathy
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DOI: 10.1093/brain/awz313
Abstract: TDP-43 is a DNA/RNA binding protein, but whether its interactions with RNA are relevant to inclusion formation in ALS is unclear. Chen et al. report that mutated forms of TDP-43 that are unable to bind… read more here.
Keywords: mutations disrupt; tardbp mutations; tdp; rrm adjacent ... See more keywords