Purification, characterization, and application of a high activity 3-ketosteroid-Δ1-dehydrogenase from Mycobacterium neoaurum DSM 1381
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09988-5
Abstract: AbstractΔ1-Dehydrogenation is one of the most important reactions for steroid drug modification. Numerous 3-ketosteroid-Δ1-dehydrogenases (KstDs) catalyzing this reaction were observed in various organisms. However, only a few have been characterized and used for substrate conversion.… read more here.
Keywords: neoaurum dsm; mycobacterium neoaurum; purification characterization; high activity ... See more keywords
Efficient production of androstenedione by repeated batch fermentation in waste cooking oil media through regulating NAD+/NADH ratio and strengthening cell vitality of Mycobacterium neoaurum.
Sign Up to like & getrecommendations! Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2019.01.144
Abstract: The bioprocess for producing androstenedione (AD) from phytosterols by using Mycobacterium neoaurum is hindered by nicotinamide adenine dinucleotides (NAD+ and NADH) ratio imbalance, insoluble substrate, and lengthy biotransformation period. This study aims to improve the… read more here.
Keywords: nad nadh; cooking oil; waste cooking; mycobacterium neoaurum ... See more keywords
Mycobacterium neoaurum line-related bacteremia with pulmonary involvement: Case report and review of literature
Sign Up to like & getrecommendations! Published in 2018 at "IDCases"
DOI: 10.1016/j.idcr.2018.01.004
Abstract: Mycobacterium neoaurum is a rapidly growing non-tuberculous mycobacterium which is ubiquitous in nature. While it can cause line related infections in immunocompromised host, case reports of urinary tract infections, cutaneous infections, pulmonary infections, and meningoencephalitis… read more here.
Keywords: line related; line; mycobacterium neoaurum; case ... See more keywords
Metabolic Adaptation of Mycobacterium neoaurum ATCC 25795 in the Catabolism of Sterols for Producing Important Steroid Intermediates.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.8b04777
Abstract: To understand the adaptation of Mycobacterium neoaurum ATCC25795 ( Mn) in sterol catabolism and steroid production, we used integrated transcriptome and proteome analysis to identify the biochemical pathways utilized in this process. Metabolic alterations during… read more here.
Keywords: catabolism; adaptation mycobacterium; mycobacterium neoaurum;
The Sterol Carrier Hydroxypropyl-β-Cyclodextrin Enhances the Metabolism of Phytosterols by Mycobacterium neoaurum
Sign Up to like & getrecommendations! Published in 2020 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00441-20
Abstract: Phytosterols from soybean are low-cost by-products of soybean oil production and, owing to their good bioavailability in mycobacteria, are preferred as the substrates for steroid drug production via biotransformation by Mycobacterium. However, the low level… read more here.
Keywords: sterol; metabolism; production; mycobacterium neoaurum ... See more keywords
Whole-Genome Analysis of Mycobacterium neoaurum DSM 1381 and the Validation of Two Key Enzymes Affecting C22 Steroid Intermediates in Sterol Metabolism
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24076148
Abstract: Mycobacterium neoaurum DSM 1381 originated from Mycobacterium neoaurum ATCC 25790 by mutagenesis screening is a strain of degrading phytosterols and accumulating important C22 steroid intermediates, including 22-hydroxy-23, 24-bisnorchola-4-en-3-one (4-HP) and 22-hydroxy-23, 24-bisnorchola-1,4-dien-3-one (HPD). However, the… read more here.
Keywords: dsm 1381; steroid intermediates; neoaurum dsm; mycobacterium neoaurum ... See more keywords