A simple strategy to differentiate between H+- and Na+-transporting NADH:quinone oxidoreductases.
Sign Up to like & getrecommendations! Published in 2020 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2020.108266
Abstract: We describe here a simple strategy to characterize transport specificity of NADH:quinone oxidoreductases, using Na+-translocating (NQR) and H+-translocating (NDH-1) enzymes of the soil bacterium Azotobacter vinelandii as the models. Submillimolar concentrations of Na+ and Li+… read more here.
Keywords: quinone oxidoreductases; simple strategy; nadh quinone; deficient ... See more keywords
Steric hindrance controls pyridine nucleotide specificity of a flavin-dependent NADH:quinone oxidoreductase.
Sign Up to like & getrecommendations! Published in 2019 at "Protein Science"
DOI: 10.2210/pdb6e2a/pdb
Abstract: The crystal structure of the NADH:quinone oxidoreductase PA1024 has been solved in complex with NAD+ to 2.2 A resolution. The nicotinamide C4 is 3.6 A from the FMN N5 atom, with a suitable orientation for… read more here.
Keywords: specificity; steric hindrance; quinone oxidoreductase; hindrance controls ... See more keywords