From the Amelioration of a NADP+‐dependent Formate Dehydrogenase to the Discovery of a New Enzyme: Round Trip from Theory to Practice
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DOI: 10.1002/cctc.201902089
Abstract: NADP+‐dependent formate dehydrogenases (FDHs) are biotechnologically relevant enzymes for cofactors regeneration in industrial processes employing redox biocatalysts. Their effective applicability is however hampered by the low cofactor and substrate affinities of the few enzymes described… read more here.
Keywords: dependent formate; nadp dependent; formate dehydrogenase; enzyme ... See more keywords
Cloning, expression, and biochemical characterization of a novel NADP+-dependent 7α-hydroxysteroid dehydrogenase from Clostridium difficile and its application for the oxidation of bile acids.
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DOI: 10.1016/j.enzmictec.2016.12.006
Abstract: A gene encoding a novel 7α-specific NADP+-dependent hydroxysteroid dehydrogenase from Clostridium difficile was cloned and heterologously expressed in Escherichia coli. The enzyme was purified using an N-terminal hexa-his-tag and biochemically characterized. The optimum temperature is… read more here.
Keywords: clostridium difficile; nadp dependent; clostridium; dehydrogenase clostridium ... See more keywords
NADP(H)-dependent biocatalysis without adding NADP(H)
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2214123120
Abstract: Significance Within cells, enzymes and cofactors catalyzing multistep processes (cascades) are often confined together—either in enclosures (e.g., organelles) or via physical association (metabolons). Nanoconfinement, offering potential general advantages for catalysis, can also be achieved by… read more here.
Keywords: catalysis; adding nadp; nadp dependent; without adding ... See more keywords
Isocitrate protects DJ-1 null dopaminergic cells from oxidative stress through NADP+-dependent isocitrate dehydrogenase (IDH)
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DOI: 10.1371/journal.pgen.1006975
Abstract: DJ-1 is one of the causative genes for early onset familiar Parkinson’s disease (PD) and is also considered to influence the pathogenesis of sporadic PD. DJ-1 has various physiological functions which converge on controlling intracellular… read more here.
Keywords: dependent isocitrate; isocitrate; nadp dependent; idh ... See more keywords
NADP-Dependent Malic Enzyme 1 Participates in the Abscisic Acid Response in Arabidopsis thaliana
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DOI: 10.3389/fpls.2018.01637
Abstract: Arabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic (NADP-ME4) NADP-dependent malic enzymes. NADP-ME2 and -ME4 show constitutive expression, in contrast to NADP-ME1 and -ME3, which are restricted to particular tissues. Here, we show that… read more here.
Keywords: seed; response; arabidopsis thaliana; nadp me1 ... See more keywords