Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy
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DOI: 10.1038/s41598-021-89987-7
Abstract: Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary. We propose to use polarization-dependent fluorescence correlation spectroscopy (Pol-FCS)… read more here.
Keywords: diffusion; evaluation; spectroscopy; nano environment ... See more keywords