Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
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DOI: 10.1021/acs.analchem.6b02528
Abstract: Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (Kd) for protein–peptide interactions can be inferred. With the current platform, four titrations… read more here.
Keywords: fluorescence; fluorescence anisotropy; anisotropy measurements; individual nanoliter ... See more keywords
Amyloid Formation in Nanoliter Droplets
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DOI: 10.3390/ijms23105480
Abstract: Processes that monitor the nucleation of amyloids and characterize the formation of amyloid fibrils are vital to medicine and pharmacology. In this study, we observe the nucleation and formation of lysozyme amyloid fibrils using a… read more here.
Keywords: amyloid formation; nucleation growth; formation; nanoliter droplets ... See more keywords