Sequence-Specific Detection of MicroRNAs Related to Clear Cell Renal Cell Carcinoma at fM Concentration by an Electroosmotically Driven Nanopore-Based Device.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b01944
Abstract: MicroRNAs (miRs) are small noncoding RNAs that play a critical role in gene regulation. Recently, traces of cancer-related miRs have been identified in body fluids, which make them remarkable noninvasive biomarkers. In this study, a… read more here.
Keywords: cell renal; detection; nanopore based; related clear ... See more keywords
Nanopore-Based Fingerprint Immunoassay Based on Rolling Circle Amplification and DNA Fragmentation.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.2c09889
Abstract: In recent years, nanopore-based sequencers have become robust tools with unique advantages for genomics applications. However, progress toward applying nanopores as highly sensitive, quantitative diagnostic tools has been impeded by several challenges. One major limitation… read more here.
Keywords: dna; rolling circle; circle amplification; detection ... See more keywords
A Nanopore Based Molnupiravir Sensor.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c00447
Abstract: Nucleoside analogues are reagents that resemble the structure of natural nucleosides and are widely applied in antiviral and anticancer therapy. Molnupiravir, a recently reported nucleoside analogue drug, has shown its inhibitory effect against SARS-CoV-2. Rapid… read more here.
Keywords: molnupiravir sensor; pharmacology; based molnupiravir; molnupiravir ... See more keywords
Nanopore-Based Protein Identification.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c11758
Abstract: The implementation of a reliable, rapid, inexpensive, and simple method for whole-proteome identification would greatly benefit cell biology research and clinical medicine. Proteins are currently identified by cleaving them with proteases, detecting the polypeptide fragments… read more here.
Keywords: identification; polypeptide fragments; based protein; protein identification ... See more keywords
Nanopore-based single molecule sequencing of the D4Z4 array responsible for facioscapulohumeral muscular dystrophy
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-13712-6
Abstract: Subtelomeric macrosatellite repeats are difficult to sequence using conventional sequencing methods owing to the high similarity among repeat units and high GC content. Sequencing these repetitive regions is challenging, even with recent improvements in sequencing… read more here.
Keywords: muscular dystrophy; facioscapulohumeral muscular; nanopore based; d4z4 array ... See more keywords
Nanopore-based measurement of the interaction of P450cam monooxygenase and putidaredoxin at single-molecule level
Sign Up to like & getrecommendations! Published in 2021 at "Faraday Discussions"
DOI: 10.1039/d1fd00042j
Abstract: Protein-protein interactions occur in a wide range of biological processes and are of great significance to life function. Characterization of transient protein-protein interactions remains a significant barrier to our understanding... read more here.
Keywords: protein; measurement interaction; based measurement; interaction p450cam ... See more keywords
Advances of nanopore-based sensing techniques for contaminants evaluation of food and agricultural products.
Sign Up to like & getrecommendations! Published in 2022 at "Critical reviews in food science and nutrition"
DOI: 10.1080/10408398.2022.2085238
Abstract: Food safety assurance systems are becoming more stringent in response to the growing food safety problems. Rapid, sensitive, and reliable detection technology is a prerequisite for the establishment of food safety assurance systems. Nanopore technology… read more here.
Keywords: food safety; food; based sensing; sensing techniques ... See more keywords
Profiling of circular RNA N6 -methyladenosine in moso bamboo (Phyllostachys edulis) using nanopore-based direct RNA sequencing.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of integrative plant biology"
DOI: 10.1111/jipb.13002
Abstract: N6 -methyladenosine (m6 A) is a prevalent modification in messenger RNAs and circular RNAs that plays important roles in regulating various aspects of RNA metabolism. However, the occurrence of the m6 A modification in plant… read more here.
Keywords: circular rnas; using nanopore; based direct; rna ... See more keywords
Nanopore-based metagenomic sequencing: a diagnostic tool in respiratory tract infection
Sign Up to like & getrecommendations! Published in 2022 at "ERJ Open Research"
DOI: 10.1183/23120541.00461-2022
Abstract: We read the recent ERJ Open Research article “Advances in diagnostic tools for respiratory tract infections: from tuberculosis to COVID-19 – changing paradigms?” by Stojanovic et al. [1] with great interest. The article represents a… read more here.
Keywords: based metagenomic; tract infection; respiratory tract; metagenomic sequencing ... See more keywords
New approach for genomic characterisation of equine sarcoid-derived BPV-1/-2 using nanopore-based sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Virology Journal"
DOI: 10.1186/s12985-021-01735-5
Abstract: Background Bovine papillomavirus (BPV) types 1 and 2 play a central role in the etiology of the most common neoplasm in horses, the equine sarcoid. The unknown mechanism behind the unique variety in clinical presentation… read more here.
Keywords: derived bpv; equine sarcoid; nanopore based; sarcoid ... See more keywords
Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in Archaea.
Sign Up to like & getrecommendations! Published in 2023 at "RNA"
DOI: 10.1261/rna.079636.123
Abstract: Ribosomal RNA (rRNA) maturation in archaea is a complex multi-step process that requires well-defined endo- and exoribonuclease activities to generate fully mature linear rRNAs. However, technical challenges prevented detailed mapping of rRNA processing steps and… read more here.
Keywords: rrna maturation; rna; rrna intermediates; rrna ... See more keywords