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Published in 2021 at "Journal of medical virology"
DOI: 10.1002/jmv.27336
Abstract: Genomic surveillance of SARS-CoV-2 plays an important role in COVID-19 pandemic control and elimination efforts, especially by elucidating its global transmission network and illustrating its viral evolution. The deployment of multiplex PCR assays that target…
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Keywords:
depth;
long amplicon;
nanopore sequencing;
sars cov ... See more keywords
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Published in 2023 at "Molecular genetics & genomic medicine"
DOI: 10.1002/mgg3.2164
Abstract: BACKGROUND The widespread adoption of exome sequencing has greatly increased the rate of genetic diagnosis for inherited conditions. However, the detection and validation of large deletions remains challenging. While numerous bioinformatics approaches have been developed…
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Keywords:
short read;
characterisation;
targeted nanopore;
read sequencing ... See more keywords
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Published in 2023 at "Prenatal Diagnosis"
DOI: 10.1002/pd.6331
Abstract: N/A This article is protected by copyright. All rights reserved.
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Keywords:
sequencing cell;
cell free;
fragment nanopore;
nanonipt short ... See more keywords
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Published in 2017 at "Acta Neuropathologica"
DOI: 10.1007/s00401-017-1743-5
Abstract: Molecular classification of cancer has entered clinical routine to inform diagnosis, prognosis, and treatment decisions. At the same time, new tumor entities have been identified that cannot be defined histologically. For central nervous system tumors,…
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Keywords:
diagnosis;
genomic epigenomic;
time;
nanopore sequencing ... See more keywords
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Published in 2021 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-021-02604-0
Abstract: The MinION nanopore sequencing device (Oxford Nanopore Technologies, Oxford, UK) is the smallest commercially available sequencer and can be used outside of conventional laboratories. The use of the MinION for forensic applications, however, is hindered…
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Keywords:
using verogen;
forenseq dna;
strs;
minion ... See more keywords
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Published in 2017 at "Carbon"
DOI: 10.1016/j.carbon.2017.01.012
Abstract: DNA sequencing using nanopores, so-named nanopore sequencing, is one of the most promising and revolutionary DNA sequencing technologies to provide the order and the type of nucleobases in a DNA molecule. In compare to those…
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Keywords:
carbon;
dna sequencing;
nanocarbons dna;
nanopore sequencing ... See more keywords
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Published in 2021 at "Nano letters"
DOI: 10.1021/acs.nanolett.1c02371
Abstract: Diverse functions of proteins, including synthesis, catalysis, and signaling, result from their highly variable amino acid sequences. The technology allowing for direct analysis of protein sequences, however, is still unsatisfactory. Recent developments of nanopore sequencing…
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Keywords:
molecule ratcheting;
motion;
single molecule;
motion peptides ... See more keywords
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Published in 2022 at "Nano letters"
DOI: 10.1021/acs.nanolett.2c01833
Abstract: O6-Carboxymethylguanosine (O6-CMG), O6-methylguanosine (O6-MeG), and abasic site (AP site) are DNA lesions induced by alkylating agents. Identification of these lesions in DNA may aid in understanding their relevance to carcinogenesis and may be used for…
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Keywords:
stalling kinetics;
dna;
dna lesions;
different dna ... See more keywords
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Published in 2021 at "ACS sensors"
DOI: 10.1021/acssensors.1c01212
Abstract: Cisplatin, which selectively binds to N7 atoms of purines to inhibit normal replication and transcription, is a widely applied chemotherapeutic drug in the treatment of cancer. Though direct identification of cisplatin lesions on DNA is…
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Keywords:
sequencing accurately;
dna;
cisplatin lesions;
cisplatin ... See more keywords
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Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00126
Abstract: Recombinant DNA is a fundamental tool in biotechnology and medicine. These DNA sequences are often built, replicated, and delivered in the form of plasmids. Validation of these plasmid sequences is a critical and time-consuming step,…
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Keywords:
plasmid;
nanopore sequencing;
long read;
biology ... See more keywords
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Published in 2018 at "Nature Biotechnology"
DOI: 10.1038/nbt.4060
Abstract: We report the sequencing and assembly of a reference genome for the human GM12878 Utah/Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore sequencer. 91.2 Gb of sequence data, representing ∼30× theoretical coverage, were…
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Keywords:
ultra long;
human genome;
nanopore sequencing;
long reads ... See more keywords