Transcription regulation through nascent RNA folding.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.166975
Abstract: Folding of RNA into secondary structures through intramolecular base pairing determines an RNA's three-dimensional architecture and associated function. Simple RNA structures like stem loops can provide specialized functions independent of coding capacity, such as protein… read more here.
Keywords: transcription; rna; regulation nascent; nascent rna ... See more keywords
Temporal Labeling of Nascent RNA Using Photoclick Chemistry in Live Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b03121
Abstract: We report the first cellular application of a photoclick SPAAC reagent to label azide-functionalized RNA. 350 nm irradiation of a cyclopropenone caged oxo-dibenzocyclooctyne (photo-ODIBO) biotin yields formation of the SPAAC reactive species, which rapidly forms… read more here.
Keywords: photo odibo; rna; chemistry; nascent rna ... See more keywords
Revealing nascent RNA processing dynamics with nano-COP.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-020-00469-y
Abstract: During maturation, eukaryotic precursor RNAs undergo processing events including intron splicing, 3'-end cleavage, and polyadenylation. Here we describe nanopore analysis of co-transcriptional processing (nano-COP), a method for probing the timing and patterns of RNA processing.… read more here.
Keywords: transcription; rna; nascent rna; nano cop ... See more keywords
FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00581-7
Abstract: Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of these dynamic processes on one transcript. We recently developed a full-length nascent RNA sequencing method in the model… read more here.
Keywords: length; rna; nascent rna; flep seq ... See more keywords
Dicer dependent tRNA derived small RNAs promote nascent RNA silencing
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac022
Abstract: Abstract In mammalian cells, small non-coding RNAs (sncRNAs) negatively regulate gene expression in a pathway known as RNA interference (RNAi). RNAi can be categorized into post-transcriptional gene silencing (PTGS), which involves the cleavage of target… read more here.
Keywords: nascent rna; small rnas; trna derived; gene ... See more keywords
RNA editing in nascent RNA affects pre-mRNA splicing.
Sign Up to like & getrecommendations! Published in 2018 at "Genome research"
DOI: 10.1101/gr.231209.117
Abstract: In eukaryotes, nascent RNA transcripts undergo an intricate series of RNA processing steps to achieve mRNA maturation. RNA editing and alternative splicing are two major RNA processing steps that can introduce significant modifications to the… read more here.
Keywords: rna affects; editing nascent; rna; nascent rna ... See more keywords
Snapshots of nascent RNA reveal cell- and stimulus-specific responses to acute kidney injury
Sign Up to like & getrecommendations! Published in 2022 at "JCI Insight"
DOI: 10.1172/jci.insight.146374
Abstract: The current strategy to detect acute injury of kidney tubular cells relies on changes in serum levels of creatinine. Yet serum creatinine (sCr) is a marker of both functional and pathological processes and does not… read more here.
Keywords: cell; kidney; snapshots nascent; injury ... See more keywords
Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing
Sign Up to like & getrecommendations! Published in 2023 at "Life Science Alliance"
DOI: 10.26508/lsa.202302054
Abstract: Dicer-dependent small non-coding vault RNA1-2 employs nascent RNA silencing to modulate the expression of a group of genes associated with membrane function. Gene expression can be regulated by transcriptional or post-transcriptional gene silencing. Recently, we… read more here.
Keywords: vault rna1; vault; expression; membrane ... See more keywords