Native Mass Spectrometry for the Study of PROTAC GNE‐987‐Containing Ternary Complexes
Sign Up to like & getrecommendations! Published in 2021 at "Chemmedchem"
DOI: 10.1002/cmdc.202100113
Abstract: PROteolysis TArgeting Chimeras (PROTACs) promote the degradation, rather than inhibition, of a drug target as a mechanism for therapeutic treatment. Bifunctional PROTAC molecules allow simultaneous binding of both the target protein and an E3‐Ubiquitin ligase,… read more here.
Keywords: gne 987; native mass; ternary complexes; mass spectrometry ... See more keywords
Rapid analysis of titer, aggregate and intact mass of antibody therapeutics using automated multi-dimensional liquid chromatography coupled with native mass spectroscopy.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of separation science"
DOI: 10.1002/jssc.202201050
Abstract: Monoclonal antibodies are tetrameric complex proteins, primarily produced using mammalian cell culture. Attributes such as titre, aggregates, and intact mass analysis are monitored during process development/ optimization. In the present study, a novel workflow such… read more here.
Keywords: native mass; spectroscopy; analysis; intact mass ... See more keywords
Exploring Phosphoinositide Binding Using Native Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1142-5_11
Abstract: Phosphoinositides interact with proteins to fulfill various functions in the cell. In many cases, they specifically recruit peripheral membrane proteins to biological membranes. The analysis of their interactions with proteins is therefore essential for understanding… read more here.
Keywords: native mass; membrane proteins; mass spectrometry; mass ... See more keywords
Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7759-8_9
Abstract: Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of… read more here.
Keywords: macromolecular complexes; characterizing intact; native mass; mass spectrometry ... See more keywords
Native mass spectrometry of human carbonic anhydrase I and its inhibitor complexes
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biological Inorganic Chemistry"
DOI: 10.1007/s00775-020-01818-8
Abstract: Abstract Native mass spectrometry is a potent technique to study and characterize biomacromolecules in their native state. Here, we have applied this method to explore the solution chemistry of human carbonic anhydrase I (hCA I)… read more here.
Keywords: carbonic anhydrase; chemistry; native mass; mass spectrometry ... See more keywords
Native Mass Spectrometry, Ion mobility, and Collision-Induced Unfolding Categorize Malaria Antigen/Antibody Binding
Sign Up to like & getrecommendations! Published in 2017 at "Journal of The American Society for Mass Spectrometry"
DOI: 10.1007/s13361-017-1782-0
Abstract: AbstractPlasmodium vivax Duffy Binding Protein (PvDBP) is a promising vaccine candidate for P. vivax malaria. Recently, we reported the epitopes on PvDBP region II (PvDBP-II) for three inhibitory monoclonal antibodies (2D10, 2H2, and 2C6). In… read more here.
Keywords: native mass; antibody; antigen antibody; mass spectrometry ... See more keywords
MetaUniDec: High-Throughput Deconvolution of Native Mass Spectra
Sign Up to like & getrecommendations! Published in 2018 at "Journal of The American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-1951-9
Abstract: AbstractThe expansion of native mass spectrometry (MS) methods for both academic and industrial applications has created a substantial need for analysis of large native MS datasets. Existing software tools are poorly suited for high-throughput deconvolution… read more here.
Keywords: native mass; metaunidec; mass; deconvolution ... See more keywords
Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM.
Sign Up to like & getrecommendations! Published in 2020 at "Structure"
DOI: 10.1016/j.str.2020.11.001
Abstract: Recent advances in single-particle cryogenic electron microscopy (cryo-EM) have enabled the structural determination of numerous protein assemblies at high resolution, yielding unprecedented insights into their function. However, despite its extraordinary capabilities, cryo-EM remains time-consuming and… read more here.
Keywords: native mass; mass spectrometry; mass; single particle ... See more keywords
How can native mass spectrometry contribute to characterization of biomacromolecular higher-order structure and interactions?
Sign Up to like & getrecommendations! Published in 2018 at "Methods"
DOI: 10.1016/j.ymeth.2018.04.025
Abstract: Native mass spectrometry (MS) is an emerging approach for characterizing biomacromolecular structure and interactions under physiologically relevant conditions. In native MS measurement, intact macromolecules or macromolecular complexes are directly ionized from a non-denaturing solvent, and… read more here.
Keywords: native mass; characterization biomacromolecular; mass spectrometry; structure interactions ... See more keywords
FAIMS and native mass spectrometry: Analysis of intact protein assemblies and protein complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c00649
Abstract: High-field asymmetric waveform ion mobility spectrometry (FAIMS) enables the separation of ions on the basis of their differential mobility in an asymmetric oscillating electric field. We, and others, have previously demonstrated the benefits of FAIMS… read more here.
Keywords: native mass; spectrometry; mass spectrometry; analysis ... See more keywords
Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI
Sign Up to like & getrecommendations! Published in 2021 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.0c05277
Abstract: Previously, we have demonstrated native mass spectrometry imaging (native MSI) in which the spatial distribution of proteins maintained in their native-like, folded conformations was determined using liquid extraction surface analysis (LESA). While providing an excellent… read more here.
Keywords: spectrometry imaging; nano desi; native mass; mass spectrometry ... See more keywords