Human Behavior-Inspired Linchpin-Directed Catalysis for Traceless Precision Labeling of Lysine in Native Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.2c00454
Abstract: The complex social ecosystem regulates the spectrum of human behavior. However, it becomes relatively easier to understand if we disintegrate the contributing factors, such as locality and interacting partners. Interestingly, it draws remarkable similarity with… read more here.
Keywords: chemistry; native proteins; human behavior; traceless ... See more keywords
A Guanidinium-Rich Polymer for Efficient Cytosolic Delivery of Native Proteins.
Sign Up to like & getrecommendations! Published in 2019 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.8b00753
Abstract: Cytosolic protein delivery is critical for the development of protein-based therapeutics. However, an efficient and robust carrier that can deliver native proteins without biological or chemical modifications into cells is highly desired. Here, we developed… read more here.
Keywords: protein; rich polymer; native proteins; guanidinium rich ... See more keywords
Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b10106
Abstract: Protein conformations play crucial roles in most, if not all, biological processes. Here we show that the current carried through a nanopore by ions allows monitoring conformational changes of single and native substrate-binding domains (SBD)… read more here.
Keywords: protein; native proteins; real time; orientation ... See more keywords
Single-Site Labeling of Native Proteins Enabled by a Chemoselective and Site-Selective Chemical Technology.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b10490
Abstract: Chemical biology research often requires precise covalent attachment of labels to the native proteins. Such methods are sought after to probe, design, and regulate the properties of proteins. At present, this demand is largely unmet… read more here.
Keywords: native proteins; site selective; labeling native; site ... See more keywords
Conformation switching of single native proteins revealed by nanomechanical probing without a pulling force.
Sign Up to like & getrecommendations! Published in 2019 at "Nanoscale"
DOI: 10.1039/c9nr01448a
Abstract: Protein conformational changes are essential to biological function, and the heterogeneous nature of the corresponding protein states provokes an interest to measure conformational changes at the single molecule level. Here we demonstrate that conformational changes… read more here.
Keywords: protein; native proteins; single native; conformation switching ... See more keywords
Decorated networks of native proteins: nanomaterials with tunable mesoscopic domain size†
Sign Up to like & getrecommendations! Published in 2021 at "Soft Matter"
DOI: 10.1039/d0sm02269a
Abstract: Natural and artificial proteins with designer properties and functionalities offer unparalleled opportunity for functional nanoarchitectures formed through self-assembly. However, to exploit this potential we need to design the system such that assembly results in desired… read more here.
Keywords: native proteins; networks native; nanomaterials tunable; domain ... See more keywords
SAT0012 ANTIBODY REACTIVITY AGAINST NATIVE PROTEINS IN RHEUMATOID ARTHRITIS
Sign Up to like & getrecommendations! Published in 2020 at "Annals of the Rheumatic Diseases"
DOI: 10.1136/annrheumdis-2020-eular.6124
Abstract: The majority of patients with rheumatoid arthritis (RA) produce autoantibodies against proteins that have undergone post-translational modfication, e.g. citrullination or carbamylation. There is growing evidence of their relevance and their potential utility to improve diagnosis,… read more here.
Keywords: anti ccp; native proteins; none declared; reactivity ... See more keywords
Western blotting of native proteins from agarose gels.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2022-0012
Abstract: We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provided native structures… read more here.
Keywords: proteins agarose; native proteins; agarose gels; blotting native ... See more keywords
Optical Fluorescence Imaging of Native Proteins Using a Fluorescent Probe with a Cell-Membrane-Permeable Carboxyl Group
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23105841
Abstract: Although various methods for selective protein tagging have been established, their ap plications are limited by the low fluorescent tagging efficiency of specific terminal regions of the native proteins of interest (NPIs). In this study,… read more here.
Keywords: fluorescence imaging; cell; native proteins; carboxyl group ... See more keywords