Accurate detection of m6A RNA modifications in native RNA sequences
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-11713-9
Abstract: The epitranscriptomics field has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here, we show that using direct RNA… read more here.
Keywords: modifications native; rna; rna modifications; m6a rna ... See more keywords
Native RNA sequencing in fission yeast reveals frequent alternative splicing isoforms
Sign Up to like & getrecommendations! Published in 2022 at "Genome Research"
DOI: 10.1101/gr.276516.121
Abstract: The unicellular yeast Schizosaccharomyces pombe (fission yeast) retains many of the splicing features observed in humans and is thus an excellent model to study the basic mechanisms of splicing. Nearly half the genes contain introns,… read more here.
Keywords: rna sequencing; alternative splicing; yeast; fission yeast ... See more keywords
Exploring the epitranscriptome by native RNA sequencing
Sign Up to like & getrecommendations! Published in 2022 at "RNA"
DOI: 10.1261/rna.079404.122
Abstract: Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput… read more here.
Keywords: rna sequencing; rna; exploring epitranscriptome; rna modifications ... See more keywords
Native RNA or cDNA Sequencing for Transcriptomic Analysis: A Case Study on Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.842299
Abstract: Direct sequencing of single molecules through nanopores allows for accurate quantification and full-length characterization of native RNA or complementary DNA (cDNA) without amplification. Both nanopore-based native RNA and cDNA approaches involve complex transcriptome procedures at… read more here.
Keywords: cdna sequencing; rna sequencing; rna; cdna ... See more keywords